| Literature DB >> 12505630 |
S Bidawid1, N Malik, O Adegbunrin, S A Sattar, J M Farber.
Abstract
Feline calicivirus (FCV) has been used by researchers as a surrogate for Norwalk virus (NV), since they share a similar genomic organization, physicochemical characteristics, and are grouped in the same family, Caliciviridae. Unlike NV, however, FCV can grow in established cell lines and produce a syncytial form of cytopathic effect. In this report, we describe the development and standardization of a plaque assay for FCV using monolayers of an established line of feline kidney (CrFK) cells in 12-well cell culture plates. The assay method has demonstrated reproducibility, ease of performance and resulted in clear plaque zones, readable in 24 h after virus inoculation. The infectivity titre of the virus by this plaque assay agreed well with tissue culture infectious dose(50) (TCID(50)) determinations. The described plaque assay would be a valuable tool in conducting various quantitative investigations using FCV as a model for NV and Norwalk-like viruses (NLV).Entities:
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Year: 2003 PMID： 12505630 DOI： 10.1016/s0166-0934(02)00214-8
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014