PURPOSE: To investigate the effect of an anti-MIF antibody on PMSG-hCG-induced murine follicular growth and ovulation and to determine whether MIF plays an essential role in this process. METHODS: Mice were primed with an intraperitoneal injection of pregnant mare serum gonadotropin (PMSG) and were treated with an anti-rat MIF antibody and human chorionic gonadotropin (hCG) to induce ovulation. After that, the ovulated ova were counted. The ovaries were studied using standard histological procedures. RESULTS: Ovaries treated with the anti-MIF antibody showed reduced numbers of growing follicles surrounded by granulosa cells and theca cells with a little proliferation compared with the control. The average numbers of ova collected from mice treated with the anti-MIF antibody were reduced compared with those collected from control mice. CONCLUSIONS: Anti-MIF antibody inhibits the follicular growth and ovulation in mice, and MIF may play an important role in the inflammatory reactions during follicle growth and ovulation.
PURPOSE: To investigate the effect of an anti-MIF antibody on PMSG-hCG-induced murine follicular growth and ovulation and to determine whether MIF plays an essential role in this process. METHODS:Mice were primed with an intraperitoneal injection of pregnant mare serum gonadotropin (PMSG) and were treated with an anti-ratMIF antibody and human chorionic gonadotropin (hCG) to induce ovulation. After that, the ovulated ova were counted. The ovaries were studied using standard histological procedures. RESULTS:Ovaries treated with the anti-MIF antibody showed reduced numbers of growing follicles surrounded by granulosa cells and theca cells with a little proliferation compared with the control. The average numbers of ova collected from mice treated with the anti-MIF antibody were reduced compared with those collected from control mice. CONCLUSIONS: Anti-MIF antibody inhibits the follicular growth and ovulation in mice, and MIF may play an important role in the inflammatory reactions during follicle growth and ovulation.
Authors: D G Armstrong; C G Gutierrez; G Baxter; A L Glazyrin; G E Mann; K J Woad; C O Hogg; R Webb Journal: J Endocrinol Date: 2000-04 Impact factor: 4.286
Authors: Maryam Shamsi; Ali Ghazavi; Amir Mohammad Saeedifar; Ghasem Mosayebi; Sana Khajeh Pour; Ali Ganji Journal: Mol Biol Rep Date: 2022-06-25 Impact factor: 2.316
Authors: Ksenia J Groh; Victor J Nesatyy; Helmut Segner; Rik I L Eggen; Marc J-F Suter Journal: Fish Physiol Biochem Date: 2011-01-13 Impact factor: 2.794
Authors: Alexei A Sharov; Geppino Falco; Yulan Piao; Suresh Poosala; Kevin G Becker; Alan B Zonderman; Dan L Longo; David Schlessinger; Minoru Sh Ko Journal: BMC Biol Date: 2008-06-03 Impact factor: 7.431