Jack J H Bleesing1, Thomas A Fleisher. 1. Department of Laboratory Medicine, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland, USA. bleesingjacobh@uams.edu
Abstract
BACKGROUND: Differences between human and murine B cells exist at all stages of B-cell development, including the stage of memory B-cell formation. B cells in mice are identified with the pan-B-cell-specific CD45 isoform, B220. In initial studies in humans, it appeared that B220 expression did not include all B cells. This study was performed to expand on those preliminary findings. METHODS: Multiparameter flow cytometric detection of B220 expression on B cells was combined with a variety of B-cell markers. RESULTS: In contrast to mice, B220 was not a pan-B-cell marker in humans but was downregulated in the majority of B cells that acquired the human memory B-cell marker, CD27, whereas a minor memory B-cell subset remained B220(+), suggesting differences in differentiation. CONCLUSIONS: The B220 isoform in humans is developmentally regulated in humans, tied to the acquisition of a memory phenotype, and as such can be used as a differentiation-specific CD45 isoform, akin to the use of CD45 isoforms to distinguish between naive and memory T-cell subsets. Patients with immunodeficiency disorders, associated with defective memory B-cell generation and absent or reduced CD27(+) B cells, showed a corresponding lack of B220 downregulation consistent with altered differentiation of B-cell subsets.
BACKGROUND: Differences between human and murine B cells exist at all stages of B-cell development, including the stage of memory B-cell formation. B cells in mice are identified with the pan-B-cell-specific CD45 isoform, B220. In initial studies in humans, it appeared that B220 expression did not include all B cells. This study was performed to expand on those preliminary findings. METHODS: Multiparameter flow cytometric detection of B220 expression on B cells was combined with a variety of B-cell markers. RESULTS: In contrast to mice, B220 was not a pan-B-cell marker in humans but was downregulated in the majority of B cells that acquired the human memory B-cell marker, CD27, whereas a minor memory B-cell subset remained B220(+), suggesting differences in differentiation. CONCLUSIONS: The B220 isoform in humans is developmentally regulated in humans, tied to the acquisition of a memory phenotype, and as such can be used as a differentiation-specific CD45 isoform, akin to the use of CD45 isoforms to distinguish between naive and memory T-cell subsets. Patients with immunodeficiency disorders, associated with defective memory B-cell generation and absent or reduced CD27(+) B cells, showed a corresponding lack of B220 downregulation consistent with altered differentiation of B-cell subsets.
Authors: Matthew Morrow; Antonio Valentin; Richard Little; Robert Yarchoan; George N Pavlakis Journal: AIDS Res Hum Retroviruses Date: 2008-04 Impact factor: 2.205
Authors: Diana M Brainard; Edward Seung; Nicole Frahm; Annaiah Cariappa; Charles C Bailey; William K Hart; Hae-Sook Shin; Sarah F Brooks; Heather L Knight; Quentin Eichbaum; Yong-Guang Yang; Megan Sykes; Bruce D Walker; Gordon J Freeman; Shiv Pillai; Susan V Westmoreland; Christian Brander; Andrew D Luster; Andrew M Tager Journal: J Virol Date: 2009-05-06 Impact factor: 5.103
Authors: B Dai; A Y Chen; C P Corkum; R J Peroutka; A Landon; S Houng; P A Muniandy; Y Zhang; E Lehrmann; K Mazan-Mamczarz; J Steinhardt; M Shlyak; Q C Chen; K G Becker; F Livak; T I Michalak; R Talwani; R B Gartenhaus Journal: Oncogene Date: 2015-10-05 Impact factor: 9.867
Authors: Ma Michelle D Peñaranda; Ingvill Jensen; Linn G Tollersrud; Jack-Ansgar Bruun; Jorunn B Jørgensen Journal: Front Immunol Date: 2019-01-29 Impact factor: 7.561