INTRODUCTION AND AIMS: We examined the effects of a weak base, chloroquine, on the trypsinogen processing in cerulein-induced pancreatitis. METHODOLOGY: Immunofluorescence studies were performed using newly generated affinity-purified antibodies to the trypsinogen activation peptide (TAP). RESULTS: The present study showed that chloroquine pretreatment blocked intracellular TAP generation in cerulein-induced pancreatitis. CONCLUSION: These results indicate that intracellular trypsinogen activation, which plays an important role in acute pancreatitis, requires a low-pH compartment, as well as serine protease activity.
INTRODUCTION AND AIMS: We examined the effects of a weak base, chloroquine, on the trypsinogen processing in cerulein-induced pancreatitis. METHODOLOGY: Immunofluorescence studies were performed using newly generated affinity-purified antibodies to the trypsinogen activation peptide (TAP). RESULTS: The present study showed that chloroquine pretreatment blocked intracellular TAP generation in cerulein-induced pancreatitis. CONCLUSION: These results indicate that intracellular trypsinogen activation, which plays an important role in acute pancreatitis, requires a low-pH compartment, as well as serine protease activity.
Authors: Christoph F Dietrich; Noor L Bekkali; Sean Burmeister; Yi Dong; Simon M Everett; Michael Hocke; Andre Ignee; Wei On; Srisha Hebbar; Kofi Oppong; Siyu Sun; Christian Jenssen; Barbara Braden Journal: Endosc Ultrasound Date: 2022 May-Jun Impact factor: 5.275
Authors: Pranav Murthy; Aatur D Singhi; Mark A Ross; Patricia Loughran; Pedram Paragomi; Georgios I Papachristou; David C Whitcomb; Amer H Zureikat; Michael T Lotze; Herbert J Zeh Iii; Brian A Boone Journal: Front Immunol Date: 2019-01-24 Impact factor: 7.561