| Literature DB >> 12498717 |
Keishi Yamashita1, Sunil Upadhyay, Motonobu Osada, Mohammad O Hoque, Yan Xiao, Masaki Mori, Fumiaki Sato, Stephen J Meltzer, David Sidransky.
Abstract
We performed a comprehensive survey of commonly inactivated tumor suppressor genes in esophageal squamous cell carcinoma (ESCC) based on functional reactivation of epigenetically silenced tumor suppressor genes by 5-aza-2'-deoxycytidine and trichostatin A using microarrays containing 12599 genes. Among 58 genes identified by this approach, 44 (76%) harbored dense CpG islands in the promoter regions. Thirteen of twenty-two tested gene promoters were methylated in cell lines, and ten in primary ESCC accompanied by silencing at the mRNA level. Potent growth suppressive activity of three genes including CRIP-1, Apolipoprotein D, and Neuromedin U in ESCC cells was demonstrated by colony focus assays. Pharmacologic reversal of epigenetic silencing is a powerful approach for comprehensive identification of tumor suppressor genes in human cancers.Entities:
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Year: 2002 PMID： 12498717 DOI： 10.1016/s1535-6108(02)00215-5
Source DB: PubMed Journal: Cancer Cell ISSN： 1535-6108 Impact factor: 31.743