Literature DB >> 12495812

Cloning, heterologous expression, and characterization of recombinant class II cytochromes c from Rhodopseudomonas palustris.

Michele A McGuirl1, Jennifer C Lee, Julia G Lyubovitsky, Chalita Thanyakoop, John H Richards, Harry B Gray, Jay R Winkler.   

Abstract

The cytochrome (cyt) c', cyt c(556), and cyt c(2) genes from Rhodopseudomonas palustris have been cloned; recombinant cyt c' and cyt c(556) have been expressed, purified, and characterized. Unlike mitochondrial cyt c, these two proteins are structurally similar to cyt b(562), in which the heme is embedded in a four-helix bundle. The hemes in both recombinant proteins form covalent thioether links to two Cys residues. UV/vis spectra of the Fe(II) and Fe(III) states of the recombinant cyts are identical with those of the corresponding native proteins. Equilibrium unfolding measurements in guanidine hydrochloride solutions confirm that native Fe(II)-cyt c(556) is more stable than the corresponding state of Fe(III)-cyt c(556) (DeltaDeltaG(f)(o) =22 kJ/mol).

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Year:  2003        PMID： 12495812     DOI： 10.1016/s0304-4165(02)00437-3

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  10 in total

1.  Site-specific collapse dynamics guide the formation of the cytochrome c' four-helix bundle.

Authors:  Tetsunari Kimura; Jennifer C Lee; Harry B Gray; Jay R Winkler
Journal:  Proc Natl Acad Sci U S A       Date:  2006-12-19       Impact factor: 11.205

2.  Probing the cytochrome c' folding landscape.

Authors:  Ekaterina V Pletneva; Ziqing Zhao; Tetsunari Kimura; Krastina V Petrova; Harry B Gray; Jay R Winkler
Journal:  J Inorg Biochem       Date:  2007-06-21       Impact factor: 4.155

3.  During Cytochrome c Maturation CcmI Chaperones the Class I Apocytochromes until the Formation of Their b-Type Cytochrome Intermediates.

Authors:  Andreia F Verissimo; Namita P Shroff; Fevzi Daldal
Journal:  J Biol Chem       Date:  2015-05-15       Impact factor: 5.157

4.  Metal-Directed Design of Supramolecular Protein Assemblies.

Authors:  J B Bailey; R H Subramanian; L A Churchfield; F A Tezcan
Journal:  Methods Enzymol       Date:  2016-06-24       Impact factor: 1.600

5.  Helical Propensity Affects the Conformational Properties of the Denatured State of Cytochrome c'.

Authors:  Travis A Danielson; Bruce E Bowler
Journal:  Biophys J       Date:  2018-01-23       Impact factor: 4.033

6.  Early events in the folding of four-helix-bundle heme proteins.

Authors:  Jasmin Faraone-Mennella; Harry B Gray; Jay R Winkler
Journal:  Proc Natl Acad Sci U S A       Date:  2005-04-20       Impact factor: 11.205

7.  Manifestations of native topology in the denatured state ensemble of Rhodopseudomonas palustris cytochrome c'.

Authors:  Tanveer A Dar; R Dustin Schaeffer; Valerie Daggett; Bruce E Bowler
Journal:  Biochemistry       Date:  2011-01-20       Impact factor: 3.162

8.  Thermodynamics of loop formation in the denatured state of rhodopseudomonas palustris cytochrome c': scaling exponents and the reconciliation problem.

Authors:  K Sudhindra Rao; Franco O Tzul; Arwen K Christian; Tia N Gordon; Bruce E Bowler
Journal:  J Mol Biol       Date:  2009-08-06       Impact factor: 5.469

9.  Variation of the axial haem ligands and haem-binding motif as a probe of the Escherichia coli c-type cytochrome maturation (Ccm) system.

Authors:  James W A Allen; Stuart J Ferguson
Journal:  Biochem J       Date:  2003-11-01       Impact factor: 3.857

10.  The histidine of the c-type cytochrome CXXCH haem-binding motif is essential for haem attachment by the Escherichia coli cytochrome c maturation (Ccm) apparatus.

Authors:  James W A Allen; Nicholas Leach; Stuart J Ferguson
Journal:  Biochem J       Date:  2005-07-15       Impact factor: 3.857
  10 in total

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