OBJECTIVES: To compare genotypic and phenotypic HIV-1 drug resistance assays. METHODS: Protease inhibitor (PI) susceptibility was phenotypically analysed in HIV-1 isolates and recombinant viruses expressing proteases from viral isolates. Two genotypic interpretation methods were carried out in parallel. RESULTS: Entirely concordant resistance levels were shown in 5/10 (50%) highly active anti-retroviral therapy (HAART)-experienced patients, whereas minor discrepancies were observed in the remaining five patients. CONCLUSIONS: The four assays provide comparable results. The recombinant virus phenotypic assay may provide the most accurate evaluation of resistance; however, genotypic interpretation procedures are helpful for daily therapeutic decisions.
OBJECTIVES: To compare genotypic and phenotypic HIV-1 drug resistance assays. METHODS: Protease inhibitor (PI) susceptibility was phenotypically analysed in HIV-1 isolates and recombinant viruses expressing proteases from viral isolates. Two genotypic interpretation methods were carried out in parallel. RESULTS: Entirely concordant resistance levels were shown in 5/10 (50%) highly active anti-retroviral therapy (HAART)-experienced patients, whereas minor discrepancies were observed in the remaining five patients. CONCLUSIONS: The four assays provide comparable results. The recombinant virus phenotypic assay may provide the most accurate evaluation of resistance; however, genotypic interpretation procedures are helpful for daily therapeutic decisions.
Authors: Valentine Budambula; Francis O Musumba; Mark K Webale; Titus M Kahiga; Francisca Ongecha-Owuor; James N Kiarie; George A Sowayi; Aabid A Ahmed; Collins Ouma; Tom Were Journal: AIDS Res Ther Date: 2015-08-15 Impact factor: 2.250