Literature DB >> 1248859

Heterogeneity among preparations of crude trypsin used to disaggregate the human tonsil.

J K Willson, T G Pretlow, J L Zaremba, M G Brattain.   

Abstract

Crude trypsin is the agent of choice for the disaggregation of human tonsil. A great variability which is not related to specific activity exists among lots of crude trypsin. An efficient lot of crude trypsin is necessary to obtain optimal proportions of tonsillar cells for the purification of lymphocytes and plasma cells by a previously reported technique.

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Year:  1976        PMID： 1248859      PMCID： PMC1444959     

Source DB:  PubMed          Journal:  Immunology        ISSN： 0019-2805            Impact factor:   7.397


  6 in total

1.  A method for the separation of lymphocytes and plasma cells from the human palatine tonsil using sedimentation in an isokinetic gradient of Ficoll in tissue culture medium.

Authors:  J K Willson; D E Luberoff; A Pitts; T C Pretlow
Journal:  Immunology       Date:  1975-01       Impact factor: 7.397

2.  A modified spectrophotometric determination of chymotrypsin, trypsin, and thrombin.

Authors:  B C HUMMEL
Journal:  Can J Biochem Physiol       Date:  1959-12

3.  Collagenase. Its effectiveness as a dispersing agent for embryonic chick thyroid and heart.

Authors:  S R Hilfer; J M Brown
Journal:  Exp Cell Res       Date:  1971-03       Impact factor: 3.905

4.  "Birth of a culture"--source of postpartum anomalies.

Authors:  M E Kaighn
Journal:  J Natl Cancer Inst       Date:  1974-11       Impact factor: 13.506

5.  Roles of collagenases and other proteolytic enzymes in the dispersal of animal tissues.

Authors:  T Kono
Journal:  Biochim Biophys Acta       Date:  1969-04-22

6.  Studies on trypsin treatment of red cells with special reference to differences between trypsin preparations.

Authors:  H Heisto; L Jensen; F Knuds
Journal:  Vox Sang       Date:  1971-08       Impact factor: 2.144
  6 in total
  3 in total

1.  The separation of human palatine tonsillar cells in the reorienting gradient zonal rotor: a large-capacity enrichment of plasma cells.

Authors:  D F Daugherty; J A Scott; T G Pretlow
Journal:  Clin Exp Immunol       Date:  1980-11       Impact factor: 4.330

2.  Evaluation of a proteolytic enzyme mixture isolated from crude trypsins in tissue disaggregation.

Authors:  D W Speicher; R L McCarl
Journal:  In Vitro       Date:  1978-10

3.  Partial purification of human colonic carcinoma cells by sedimentation.

Authors:  M G Brattain; P M Kimball; T G Pretlow; A M Pitts
Journal:  Br J Cancer       Date:  1977-06       Impact factor: 7.640
  3 in total

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