Ling Ding1, Shu Zheng. 1. Cancer Institute, Zhejiang University, Hangzhou 310009, China.
Abstract
OBJECTIVE: To investigate the possible association of osteopontin (OPN) with liver metastasis in colorectal cancer. METHODS: RNA was quantitated by RT-PCR from colorectal cancer and surrounding normal tissues, also from liver metastatic tissues and normal liver tissues. ISH was utilized to detect OPN mRNA in colorectal cancer and liver metastatic tissues. RESULTS: In 44 cases, the expressions of OPN mRNA in colorectal cancer tissues were higher than those in the surrounding normal tissues (t = 4.89, P = 0.000). The expression of OPN mRNA in colorectal cancer was as high as 18.2%, but in liver metastasis was 80% (chi(2) = 22.42, P = 0.000). In ISH, OPN mRNA located in the cytoplasm of colorectal cancer cells. CONCLUSIONS: The expressions of OPN mRNA were the highest in liver metastatic tissues, moderate in colorectal cancer tissues, and lowest in surrounding normal tissues, indicating that OPN is a potential marker for liver metastasis of colorectal cancer.
OBJECTIVE: To investigate the possible association of osteopontin (OPN) with liver metastasis in colorectal cancer. METHODS: RNA was quantitated by RT-PCR from colorectal cancer and surrounding normal tissues, also from liver metastatic tissues and normal liver tissues. ISH was utilized to detect OPN mRNA in colorectal cancer and liver metastatic tissues. RESULTS: In 44 cases, the expressions of OPN mRNA in colorectal cancer tissues were higher than those in the surrounding normal tissues (t = 4.89, P = 0.000). The expression of OPN mRNA in colorectal cancer was as high as 18.2%, but in liver metastasis was 80% (chi(2) = 22.42, P = 0.000). In ISH, OPN mRNA located in the cytoplasm of colorectal cancer cells. CONCLUSIONS: The expressions of OPN mRNA were the highest in liver metastatic tissues, moderate in colorectal cancer tissues, and lowest in surrounding normal tissues, indicating that OPN is a potential marker for liver metastasis of colorectal cancer.
Authors: Ayman Bishay; Suhail Raoof; Adebayo Esan; Arthur Sung; Siraj Wali; Leonard Y Lee; Liziamma George; Anthony Saleh; Michael Baumann Journal: Ann Thorac Med Date: 2007-07 Impact factor: 2.219