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Literature DB >> 12486331

Preparation of dissociated zebrafish spinal neuron cultures.

Abstract

The method described here explains a simple protocol for how to prepare dissociated Zebrafish spinal neuron cultures. The neurons grow fast in a simple culture medium and at room temperature. Considering the advantages afforded by the optical transparency of the Zebrafish embryo combined with the powerful molecular perturbation techniques available, this technique has potential to further advance molecular analysis of axon growth and guidance.

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Year: 2001 PMID： 12486331 DOI： 10.1023/a:1016349232389

Source DB: PubMed Journal: Methods Cell Sci ISSN： 1381-5741

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Cited

14 in total

1. A novel approach to study motor neurons from zebrafish embryos and larvae in culture.

Authors: Stacey A Sakowski; J Simon Lunn; Angela S Busta; Madeline Palmer; James J Dowling; Eva L Feldman
Journal: J Neurosci Methods Date: 2012-01-20 Impact factor: 2.390

2. Embryonic zebrafish neuronal growth is not affected by an applied electric field in vitro.

Authors: Peter Cormie; Kenneth R Robinson
Journal: Neurosci Lett Date: 2006-11-07 Impact factor: 3.046

3. STIM1 Is Required for Remodeling of the Endoplasmic Reticulum and Microtubule Cytoskeleton in Steering Growth Cones.

Authors: Macarena Pavez; Adrian C Thompson; Hayden J Arnott; Camilla B Mitchell; Ilaria D'Atri; Emily K Don; John K Chilton; Ethan K Scott; John Y Lin; Kaylene M Young; Robert J Gasperini; Lisa Foa
Journal: J Neurosci Date: 2019-04-25 Impact factor: 6.167

4. Primary cell culture of adult zebrafish spinal neurons for electrophysiological studies.

Authors: Max E Meade; Jessica E Roginsky; Joseph R Schulz
Journal: J Neurosci Methods Date: 2019-04-24 Impact factor: 2.390

5. Zebrafish atlastin controls motility and spinal motor axon architecture via inhibition of the BMP pathway.

Authors: Coralie Fassier; James A Hutt; Steffen Scholpp; Andrew Lumsden; Bruno Giros; Fatiha Nothias; Sylvie Schneider-Maunoury; Corinne Houart; Jamilé Hazan
Journal: Nat Neurosci Date: 2010-10-10 Impact factor: 24.884

6. Ca²⁺ release via two-pore channel type 2 (TPC2) is required for slow muscle cell myofibrillogenesis and myotomal patterning in intact zebrafish embryos.

Authors: Jeffrey J Kelu; Sarah E Webb; John Parrington; Antony Galione; Andrew L Miller
Journal: Dev Biol Date: 2017-04-06 Impact factor: 3.582

Preparation of dissociated zebrafish spinal neuron cultures.

1. A novel approach to study motor neurons from zebrafish embryos and larvae in culture.

2. Embryonic zebrafish neuronal growth is not affected by an applied electric field in vitro.

3. STIM1 Is Required for Remodeling of the Endoplasmic Reticulum and Microtubule Cytoskeleton in Steering Growth Cones.

4. Primary cell culture of adult zebrafish spinal neurons for electrophysiological studies.

5. Zebrafish atlastin controls motility and spinal motor axon architecture via inhibition of the BMP pathway.

6. Ca²⁺ release via two-pore channel type 2 (TPC2) is required for slow muscle cell myofibrillogenesis and myotomal patterning in intact zebrafish embryos.

7. N-cadherin prodomain cleavage regulates synapse formation in vivo.

8. Long-Range Optogenetic Control of Axon Guidance Overcomes Developmental Boundaries and Defects.

9. Knockdown of Pnpla6 protein results in motor neuron defects in zebrafish.

10. Primary neuron culture for nerve growth and axon guidance studies in zebrafish (Danio rerio).