| Literature DB >> 12486241 |
Philippe Le Bouteiller1, Aliz Barakonyi, Jérome Giustiniani, Françoise Lenfant, Anne Marie-Cardine, Maryse Aguerre-Girr, Magali Rabot, Ivan Hilgert, Fathia Mami-Chouaib, Julie Tabiasco, Laurence Boumsell, Armand Bensussan.
Abstract
Circulating human natural killer (NK) lymphocytes have been functionally defined by their ability to exert cytotoxic activity against MHC class I-negative target cell lines, including K562. Therefore, it was proposed that NK cells recognized the "missing self." We show here that the Ig-like CD160 receptor expressed by circulating CD56(dim+) NK cells or IL-2-deprived NK cell lines is mainly involved in their cytotoxic activity against K562 target cells. Further, we report that HLA-C molecules that are constitutively expressed by K562 trigger NK cell lysis through CD160 receptor engagement. In addition, we demonstrate, with recombinant soluble HLA-Cw3 and CD160 proteins, direct interaction of these molecules. We also find that CD158b inhibitory receptors partially interfere with CD160-mediated cytotoxicity, whereas CD94CD159a and CD85j have no effect on engagement with their respective ligands. Thus, CD160HLA-C interaction constitutes a unique pathway to trigger NK cell cytotoxic activity.Entities:
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Year: 2002 PMID： 12486241 PMCID： PMC139252 DOI： 10.1073/pnas.012681099
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205