Purification of proteins susceptible to oxidation at cysteine residues: identification of malate dehydrogenase as a target for S-glutathiolation.

The cysteine residues of proteins are susceptible to a range of oxidative modifications, including S-glutathiolation. Here we present a study in which we used glutathione-agarose to purify cardiac proteins that undergo S-glutathiolation during oxidative stress. We then identified one of the oxidized proteins as malate dehydrogenase.