Literature DB >> 12485713

New label enzymes for bioluminescent enzyme immunoassay.

Masako Maeda1.   

Abstract

Firefly luciferase catalyzes the oxidation of luciferin in the presence of ATP, magnesium ion and molecular oxygen with a high quantum yield. Due to its high sensitivity and specificity for ATP, luciferase has been used for bioluminescent detection of ATP in various biological samples. But it is not known well to apply the detection of immunoassay. In this article, the use of various enzymes as labels in the design and development of immunoassays for biomolecules has been reviewed.

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Year:  2003        PMID： 12485713     DOI： 10.1016/s0731-7085(02)00514-9

Source DB:  PubMed          Journal:  J Pharm Biomed Anal        ISSN： 0731-7085            Impact factor:   3.935


  4 in total

1.  Immunoassay Methods and their Applications in Pharmaceutical Analysis: Basic Methodology and Recent Advances.

Authors:  Ibrahim A Darwish
Journal:  Int J Biomed Sci       Date:  2006-09

2.  Polychlorinated Biphenyls Induce Mitochondrial Dysfunction in SH-SY5Y Neuroblastoma Cells.

Authors:  Stefania Cocco; Agnese Secondo; Adelaide Del Viscovo; Claudio Procaccini; Luigi Formisano; Cristina Franco; Alba Esposito; Antonella Scorziello; Giuseppe Matarese; Gianfranco Di Renzo; Lorella Maria Teresa Canzoniero
Journal:  PLoS One       Date:  2015-06-23       Impact factor: 3.240

3.  Knocking-out the Siah2 E3 ubiquitin ligase prevents mitochondrial NCX3 degradation, regulates mitochondrial fission and fusion, and restores mitochondrial function in hypoxic neurons.

Authors:  Maria Josè Sisalli; Gaetano Ianniello; Claudia Savoia; Ornella Cuomo; Lucio Annunziato; Antonella Scorziello
Journal:  Cell Commun Signal       Date:  2020-03-12       Impact factor: 5.712

4.  SpyTag/SpyCatcher Cyclization Enhances the Thermostability of Firefly Luciferase.

Authors:  Meng Si; Qing Xu; Ling Jiang; He Huang
Journal:  PLoS One       Date:  2016-09-22       Impact factor: 3.240
  4 in total

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