Literature DB >> 12485712

Immobilized nicotinic receptor stationary phases: going with the flow in high-throughput screening and pharmacological studies.

Ruin Moaddel1, Irving W Wainer.   

Abstract

The nicotinic acetylcholine receptor (nAChR) subtypes alpha3beta4-nAChR and alpha4beta2-nAChR have been immobilized and the resulting stationary phases used to determine binding affinities. The alpha3beta4-nAChR column was coupled to a C(18) column and a mixture of 18 compounds was sorted into ligands and non-ligands for the alpha3beta4-nAChR. The results demonstrate that the nAChR stationary phases can be used for on-line high-throughput screening (HTS).

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Year:  2003        PMID： 12485712     DOI： 10.1016/s0731-7085(02)00513-7

Source DB:  PubMed          Journal:  J Pharm Biomed Anal        ISSN： 0731-7085            Impact factor:   3.935


  3 in total

1.  Screening of tobacco smoke condensate for nicotinic acetylcholine receptor ligands using cellular membrane affinity chromatography columns and missing peak chromatography.

Authors:  Alexandre Maciuk; Ruin Moaddel; Jun Haginaka; Irving W Wainer
Journal:  J Pharm Biomed Anal       Date:  2007-11-26       Impact factor: 3.935

2.  The synthesis and characterization of a nuclear membrane affinity chromatography column for the study of human breast cancer resistant protein (BCRP) using nuclear membranes obtained from the LN-229 cells.

Authors:  K-L Habicht; C Frazier; N Singh; R Shimmo; I W Wainer; R Moaddel
Journal:  J Pharm Biomed Anal       Date:  2012-10-08       Impact factor: 3.935

3.  Development and characterization of the α3β4α5 nicotinic receptor cellular membrane affinity chromatography column and its application for on line screening of plant extracts.

Authors:  L Ciesla; M Okine; A Rosenberg; K S S Dossou; L Toll; I W Wainer; R Moaddel
Journal:  J Chromatogr A       Date:  2015-12-29       Impact factor: 4.759
  3 in total

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