OBJECTIVE: We sought to identify differentially expressed genes in human in stent stenosis (ISS) to provide insights into the mechanism of disease. METHODS AND RESULTS: Using representation difference analysis, we examined differential gene expression between cultured normal human medial vascular smooth muscle cells (VSMCs) and cells from primary atherosclerotic plaques or ISS sites. Specific groups of genes were overexpressed in ISS and plaque VSMCs, including cell cycle regulatory proteins and cell matrix and contractile proteins. Differential expression was validated by virtual Northern analysis, reverse transcriptase-polymerase chain reaction, in situ hybridization, and immunohistochemistry. All ISS genes were expressed by normal intima and had even higher expression in primary plaque VSMCs. CONCLUSIONS: ISS VSMCs have a stable gene expression profile reflecting an intimal pattern, intermediate between normal medial and primary plaque VSMCs. Differential expression profiling may identify markers of disease that are overexpressed in ISS and also help elucidate the origin of the ISS lesion.
OBJECTIVE: We sought to identify differentially expressed genes in human in stent stenosis (ISS) to provide insights into the mechanism of disease. METHODS AND RESULTS: Using representation difference analysis, we examined differential gene expression between cultured normal human medial vascular smooth muscle cells (VSMCs) and cells from primary atherosclerotic plaques or ISS sites. Specific groups of genes were overexpressed in ISS and plaque VSMCs, including cell cycle regulatory proteins and cell matrix and contractile proteins. Differential expression was validated by virtual Northern analysis, reverse transcriptase-polymerase chain reaction, in situ hybridization, and immunohistochemistry. All ISS genes were expressed by normal intima and had even higher expression in primary plaque VSMCs. CONCLUSIONS: ISS VSMCs have a stable gene expression profile reflecting an intimal pattern, intermediate between normal medial and primary plaque VSMCs. Differential expression profiling may identify markers of disease that are overexpressed in ISS and also help elucidate the origin of the ISS lesion.
Authors: Hannes M Findeisen; Florence Gizard; Yue Zhao; Hua Qing; Elizabeth B Heywood; Karrie L Jones; Dianne Cohn; Dennis Bruemmer Journal: Arterioscler Thromb Vasc Biol Date: 2011-01-13 Impact factor: 8.311
Authors: Manuel A Pallero; Melissa Talbert Roden; Yiu-Fai Chen; Peter G Anderson; Jack Lemons; Brigitta C Brott; Joanne E Murphy-Ullrich Journal: J Vasc Res Date: 2009-12-16 Impact factor: 1.934
Authors: Kirsten Riches; Larissa Franklin; Azhar Maqbool; Michelle Peckham; Matthew Adams; Jacquelyn Bond; Philip Warburton; Nicole T Feric; Marlys L Koschinsky; David J O'Regan; Stephen G Ball; Neil A Turner; Karen E Porter Journal: Int J Biochem Cell Biol Date: 2013-05-31 Impact factor: 5.085