Ge Jin1, Ting Zhang, Tao Wang, Li-ping Yang. 1. Department of Biochemistry, Medical College, Zhengzhou University, Zhengzhou 450052, P. R. China. xinxin@371.net
Abstract
BACKGROUND & OBJECTIVE: Recently, the research showed that cinnamic acid (CINN) could inhibit some cancer cells. This study was designed to investigate the effects of alpha-interferon and CINN on proliferation of human lung cancer cells. METHODS: Human lung adenocarcinoma cell A549 were treated with alpha-interferon and CINN. The growth curve, soft-agar colony-forming rate, and methyl greenpyronine staining were used to analyze the inhibition to cell proliferation. The cell cycle was detected by flow cytometry(FCM). RESULTS: alpha-interferon and CINN could markedly inhibit proliferation of A549 (P < 0.05), promote the differentiation (P < 0.05), and reduce the colony-forming rate in the soft-agar drastically(P < 0.05), FCM showed that the cell cycle was arrested in the G1/G0 phase. CONCLUSION: alpha-interferon and CINN can inhibit the proliferation of lung adenocarcinoma cell. Combination of the alpha-interferon and CINN show the stronger effect that the alpha-interferon or CINN alone.
BACKGROUND & OBJECTIVE: Recently, the research showed that cinnamic acid (CINN) could inhibit some cancer cells. This study was designed to investigate the effects of alpha-interferon and CINN on proliferation of humanlung cancer cells. METHODS:Humanlung adenocarcinoma cell A549 were treated with alpha-interferon and CINN. The growth curve, soft-agar colony-forming rate, and methyl greenpyronine staining were used to analyze the inhibition to cell proliferation. The cell cycle was detected by flow cytometry(FCM). RESULTS: alpha-interferon and CINN could markedly inhibit proliferation of A549 (P < 0.05), promote the differentiation (P < 0.05), and reduce the colony-forming rate in the soft-agar drastically(P < 0.05), FCM showed that the cell cycle was arrested in the G1/G0 phase. CONCLUSION: alpha-interferon and CINN can inhibit the proliferation of lung adenocarcinoma cell. Combination of the alpha-interferon and CINN show the stronger effect that the alpha-interferon or CINN alone.