Literature DB >> 12477872

Contribution of the zinc finger to zinc and DNA binding by a suppressor of posttranscriptional gene silencing.

Rene Van Wezel1, Huanting Liu, Zirong Wu, John Stanley, Yiguo Hong.   

Abstract

The zinc finger C(36)-X1-C(38)-X7-C(46)-X6-H(53) of the nuclearly localized C2 protein of Tomato yellow leaf curl virus China is involved in pathogenicity and suppression of posttranscriptional gene silencing (PTGS). Here, we demonstrate that the zinc finger is indispensable for the C2 protein to bind zinc and DNA. Mutation of cysteine residue C(36), C(38), or C(46) reduced the zinc and DNA binding capacity of C2 protein. When expressed from potato virus X, all three mutants, C2-C(36)R, C2-C(38)N, and C2-C(46)I, tagged with a green fluorescent protein (GFP) were still capable of transporting GFP into but aggregated abnormally in nuclei. Our data establish that zinc- and DNA-binding activity correlates with C2-mediated pathogenesis and PTGS suppression.

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Year:  2003        PMID： 12477872      PMCID： PMC140617          DOI： 10.1128/jvi.77.1.696-700.2003

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  39 in total

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Review 3.  Gene silencing as an adaptive defence against viruses.

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4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
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5.  Mutation of three cysteine residues in Tomato yellow leaf curl virus-China C2 protein causes dysfunction in pathogenesis and posttranscriptional gene-silencing suppression.

Authors:  Wezel Rene van; Xiangli Dong; Huanting Liu; Po Tien; John Stanley; Yiguo Hong
Journal:  Mol Plant Microbe Interact       Date:  2002-03       Impact factor: 4.171

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Authors:  Y Hong; K Saunders; J Stanley
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Authors:  A. A. Sanderfoot; S. G. Lazarowitz
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Authors:  K D Kasschau; J C Carrington
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9.  Transgenes are dispensable for the RNA degradation step of cosuppression.

Authors:  J C Palauqui; H Vaucheret
Journal:  Proc Natl Acad Sci U S A       Date:  1998-08-04       Impact factor: 11.205

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  20 in total

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2.  Differential roles of AC2 and AC4 of cassava geminiviruses in mediating synergism and suppression of posttranscriptional gene silencing.

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Review 3.  Silencing suppressors: viral weapons for countering host cell defenses.

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4.  The 35-amino acid C2 protein of Cotton leaf curl Kokhran virus, Burewala, implicated in resistance breaking in cotton, retains some activities of the full-length protein.

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5.  Functional modulation of the geminivirus AL2 transcription factor and silencing suppressor by self-interaction.

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6.  Geminivirus AL2 and L2 proteins suppress transcriptional gene silencing and cause genome-wide reductions in cytosine methylation.

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7.  P2 of Rice grassy stunt virus (RGSV) and p6 and p9 of Rice ragged stunt virus (RRSV) isolates from Vietnam exert suppressor activity on the RNA silencing pathway.

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Journal:  Virus Genes       Date:  2015-07-28       Impact factor: 2.332

8.  The N-terminal 85 amino acids of the barley stripe mosaic virus gammab pathogenesis protein contain three zinc-binding motifs.

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Journal:  J Virol       Date:  2004-07       Impact factor: 5.103

9.  Short interfering RNA accumulation correlates with host recovery in DNA virus-infected hosts, and gene silencing targets specific viral sequences.

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10.  Functional characterization of the nuclear localization signal for a suppressor of posttranscriptional gene silencing.

Authors:  Xiangli Dong; Rene van Wezel; John Stanley; Yiguo Hong
Journal:  J Virol       Date:  2003-06       Impact factor: 5.103
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