Literature DB >> 12460110

Structural-functional analysis of bacteriophage T7 RNA polymerase.

V L Tunitskaya1, S N Kochetkov.   

Abstract

This review summarizes our results of the structural and functional studies of bacteriophage T7 DNA-dependent RNA polymerase (T7 RNAP). Particular features of this enzyme (the single-subunit composition, relatively low molecular weight) make it the most convenient model for investigating the physicochemical aspects of transcription. The review discusses the main properties of T7 RNAP, interaction between the enzyme and promoter, principle stages of T7-transcription, and also the results of structural and functional studies by affinity modification and both random and site-directed mutagenesis techniques.

Entities:  

Mesh：

Substances：

Year:  2002        PMID： 12460110     DOI： 10.1023/a:1020911223250

Source DB:  PubMed          Journal:  Biochemistry (Mosc)        ISSN： 0006-2979            Impact factor:   2.487


  13 in total

1.  An inducible T7 RNA polymerase-dependent plasmid system.

Authors:  Matthias Hamdorf; Heide Muckenfuss; Ulrich Tschulena; Stephan Pleschka; Ralf Sanzenbacher; Klaus Cichutek; Egbert Flory
Journal:  Mol Biotechnol       Date:  2006-05       Impact factor: 2.695

2.  A Highly Productive, One-Pot Cell-Free Protein Synthesis Platform Based on Genomically Recoded Escherichia coli.

Authors:  Benjamin J Des Soye; Vincent R Gerbasi; Paul M Thomas; Neil L Kelleher; Michael C Jewett
Journal:  Cell Chem Biol       Date:  2019-11-06       Impact factor: 8.116

3.  Coupling mutagenesis and parallel deep sequencing to probe essential residues in a genome or gene.

Authors:  William P Robins; Shah M Faruque; John J Mekalanos
Journal:  Proc Natl Acad Sci U S A       Date:  2013-02-11       Impact factor: 11.205

Review 4.  Fluorescent methods to study transcription initiation and transition into elongation.

Authors:  Aishwarya P Deshpande; Shemaila Sultana; Smita S Patel
Journal:  Exp Suppl       Date:  2014

5.  Genomic characterization of the intron-containing T7-like phage phiL7 of Xanthomonas campestris.

Authors:  Chia-Ni Lee; Juey-Wen Lin; Shu-Fen Weng; Yi-Hsiung Tseng
Journal:  Appl Environ Microbiol       Date:  2009-10-23       Impact factor: 4.792

6.  In silico design and enzymatic synthesis of functional RNA nanoparticles.

Authors:  Kirill A Afonin; Wojciech K Kasprzak; Eckart Bindewald; Maria Kireeva; Mathias Viard; Mikhail Kashlev; Bruce A Shapiro
Journal:  Acc Chem Res       Date:  2014-04-23       Impact factor: 22.384

7.  Interaction Analysis of T7 RNA Polymerase with Heparin and Its Low Molecular Weight Derivatives - An In Silico Approach.

Authors:  Subhomoi Borkotoky; Chetan Kumar Meena; Ayaluru Murali
Journal:  Bioinform Biol Insights       Date:  2016-08-29

8.  Transcription-Based Amplified Colorimetric Thrombin Sensor Using Non-Crosslinking Aggregation of DNA-Modified Gold Nanoparticles.

Authors:  Yu Muto; Gen Hirao; Tamotsu Zako
Journal:  Sensors (Basel)       Date:  2021-06-24       Impact factor: 3.576

9.  Isocost Lines Describe the Cellular Economy of Genetic Circuits.

Authors:  Andras Gyorgy; José I Jiménez; John Yazbek; Hsin-Ho Huang; Hattie Chung; Ron Weiss; Domitilla Del Vecchio
Journal:  Biophys J       Date:  2015-08-04       Impact factor: 4.033

10.  Novel group of podovirus infecting the marine bacterium Alteromonas macleodii.

Authors:  Inmaculada Garcia-Heredia; Francisco Rodriguez-Valera; Ana-Belen Martin-Cuadrado
Journal:  Bacteriophage       Date:  2013-04-01
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.