Literature DB >> 12458744

A new method for fish leucocyte counting and partial differentiation by flow cytometry.

Takamasa Inoue1, Tadaaki Moritomo, Yumi Tamura, Sachiko Mamiya, Hiroshi Fujino, Teruyuki Nakanishi.   

Abstract

A simple and rapid method for analysis of fish blood cells is presented. Carp (Cyprinus carpio) blood was diluted 200 times with Hanks' solution containing 1 microg/ml of DiOC6(3) which is a fluorescent, lipophilic dye. After staining for 10 min, the blood cells were measured by a flow cytometer (FACS). Several blood cell populations were identified by different FL-1 (green fluorescence), FSC (forward scatter), and SSC (side scatter) properties. FL-1 v. SSC or FSC v. SSC dot-plot of stained blood cells displayed five separate cell populations: erythrocytes: a mixture of thrombocytes plus lymphocytes; monocytes; neutrophils; and basophils. The number of each type of blood cell counted by the FACS was in good agreement with those counted microscopically.

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Year:  2002        PMID： 12458744     DOI： 10.1006/fsim.2002.0413

Source DB:  PubMed          Journal:  Fish Shellfish Immunol        ISSN： 1050-4648            Impact factor:   4.581


  7 in total

1.  Immune competence assessment in marine medaka (Orzyias melastigma)-a holistic approach for immunotoxicology.

Authors:  Roy R Ye; Drew R Peterson; Frauke Seemann; Shin-Ichi Kitamura; J S Lee; Terrance C K Lau; Stephen K W Tsui; Doris W T Au
Journal:  Environ Sci Pollut Res Int       Date:  2016-07-29       Impact factor: 4.223

2.  Comprehensive validation of T- and B-cell deficiency in rag1-null zebrafish: Implication for the robust innate defense mechanisms of teleosts.

Authors:  Yumie Tokunaga; Masamichi Shirouzu; Ryota Sugahara; Yasutoshi Yoshiura; Ikunari Kiryu; Mitsuru Ototake; Takahiro Nagasawa; Tomonori Somamoto; Miki Nakao
Journal:  Sci Rep       Date:  2017-08-08       Impact factor: 4.379

3.  Acute-phase proteins during inflammatory reaction by bacterial infection: Fish-model.

Authors:  Ives Charlie-Silva; Andre Klein; Juliana M M Gomes; Ed J R Prado; Alessandra C Moraes; Silas F Eto; Dayanne C Fernandes; José J Fagliari; José D Corrêa Junior; Carla Lima; Mônica Lopes-Ferreira; Katia Conceição; Wilson G Manrique; Marco A A Belo
Journal:  Sci Rep       Date:  2019-03-18       Impact factor: 4.379

4.  Zebrafish kidney phagocytes utilize macropinocytosis and Ca+-dependent endocytic mechanisms.

Authors:  Claudia Hohn; Sang-Ryul Lee; Lesya M Pinchuk; Lora Petrie-Hanson
Journal:  PLoS One       Date:  2009-02-02       Impact factor: 3.240

5.  Characterization of rag1 mutant zebrafish leukocytes.

Authors:  Lora Petrie-Hanson; Claudia Hohn; Larry Hanson
Journal:  BMC Immunol       Date:  2009-02-03       Impact factor: 3.615

6.  What the Erythrocytic Nuclear Alteration Frequencies Could Tell Us about Genotoxicity and Macrophage Iron Storage?

Authors:  Juliana M M Gomes; Heder J Ribeiro; Marcela S Procópio; Betânia M Alvarenga; Antônio C S Castro; Walderez O Dutra; José B B da Silva; José D Corrêa Junior
Journal:  PLoS One       Date:  2015-11-30       Impact factor: 3.240

7.  GAS1: A New β-Glucan Immunostimulant Candidate to Increase Rainbow Trout (Oncorhynchus mykiss) Resistance to Bacterial Infections With Aeromonas salmonicida achromogenes.

Authors:  Valérie Cornet; Trinh Dinh Khuyen; Syaghalirwa N M Mandiki; Stéphane Betoulle; Peter Bossier; Felipe E Reyes-López; Lluis Tort; Patrick Kestemont
Journal:  Front Immunol       Date:  2021-07-06       Impact factor: 7.561
  7 in total

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