BACKGROUND & AIMS: The protein expression and interactions of the ErbB receptors were examined in different liver proliferation models in vivo and in vitro, including ontogeny and regeneration following partial hepatectomy. METHODS: Expression and tyrosine phosphorylation status of specific ErbB proteins were studied by immunologic methods. RESULTS: The epidermal growth factor receptor, ErbB2, and ErbB3 were the only ErbB proteins detected in the liver parenchyma on embryonic day 19. ErbB2 disappeared by the third week after birth and could not be appreciably induced in the adult animal by partial hepatectomy. ErbB2 was also detected in multipotent stem (RLE) and hepatoma (H4IIe) cell lines as well as in fetal, but not adult, hepatocyte cultures. Only epidermal growth factor receptor and ErbB3 were detected in adult liver, and both showed circadian variation in protein expression. ErbB4 was not detected in any model. Patterns of ligand-induced ErbB phosphorylation differed between fetal and adult hepatocytes. CONCLUSIONS: Complex and independent programs regulate the ErbB receptors, with implications for differential cell signaling in hepatic development and regeneration.
BACKGROUND & AIMS: The protein expression and interactions of the ErbB receptors were examined in different liver proliferation models in vivo and in vitro, including ontogeny and regeneration following partial hepatectomy. METHODS: Expression and tyrosine phosphorylation status of specific ErbB proteins were studied by immunologic methods. RESULTS: The epidermal growth factor receptor, ErbB2, and ErbB3 were the only ErbB proteins detected in the liver parenchyma on embryonic day 19. ErbB2 disappeared by the third week after birth and could not be appreciably induced in the adult animal by partial hepatectomy. ErbB2 was also detected in multipotent stem (RLE) and hepatoma (H4IIe) cell lines as well as in fetal, but not adult, hepatocyte cultures. Only epidermal growth factor receptor and ErbB3 were detected in adult liver, and both showed circadian variation in protein expression. ErbB4 was not detected in any model. Patterns of ligand-induced ErbB phosphorylation differed between fetal and adult hepatocytes. CONCLUSIONS: Complex and independent programs regulate the ErbB receptors, with implications for differential cell signaling in hepatic development and regeneration.
Authors: Shirish Paranjpe; William C Bowen; Wendy M Mars; Anne Orr; Meagan M Haynes; Marie C DeFrances; Silvia Liu; George C Tseng; Anastasia Tsagianni; George K Michalopoulos Journal: Hepatology Date: 2016-10-01 Impact factor: 17.425
Authors: Lawrence A Scheving; Xiuqi Zhang; Oscar A Garcia; Rebecca F Wang; Mary C Stevenson; David W Threadgill; William E Russell Journal: Am J Physiol Gastrointest Liver Physiol Date: 2014-01-09 Impact factor: 4.052
Authors: Anastasia Tsagianni; Wendy M Mars; Bharat Bhushan; William C Bowen; Anne Orr; John Stoops; Shirish Paranjpe; George C Tseng; Silvia Liu; George K Michalopoulos Journal: Am J Pathol Date: 2018-07-20 Impact factor: 4.307
Authors: Lawrence A Scheving; Xiuqi Zhang; Mary C Stevenson; Michael A Weintraub; Annam Abbasi; Andrea M Clarke; David W Threadgill; William E Russell Journal: Am J Physiol Gastrointest Liver Physiol Date: 2015-10-22 Impact factor: 4.052
Authors: Nicholas J Hand; Zankhana R Master; Steven F Eauclaire; Daniel E Weinblatt; Randolph P Matthews; Joshua R Friedman Journal: Gastroenterology Date: 2008-12-09 Impact factor: 22.682
Authors: Regina Samaga; Julio Saez-Rodriguez; Leonidas G Alexopoulos; Peter K Sorger; Steffen Klamt Journal: PLoS Comput Biol Date: 2009-08-07 Impact factor: 4.475
Authors: Jie Liu; Angela Ahiekpor; Li Li; Xianxing Li; Patrick Arbuthnot; Michael Kew; Mark A Feitelson Journal: Int J Cancer Date: 2009-10-15 Impact factor: 7.396