Purification, crystallization and preliminary X-ray diffraction analysis of the carbohydrate-binding domain of flocculin, a cell-adhesion molecule from Saccharomyces carlsbergensis.

The recombinant carbohydrate-binding domain of the cell-surface lectin flocculin from brewer's yeast has been identified, purified and crystallized. The expression of the protein is associated with the nutritional state of the yeast. P2(1)2(1)2(1) crystals with unit-cell parameters a = 36.5, b = 59.7, c = 83.1 A were obtained in hanging drops at 295 K using 25%(w/v) PEG 4000, 0.05 M KH(2)PO(4) as precipitant. X-ray diffraction data have been obtained to 2.6 A. The asymmetric unit contains one molecule and has a solvent content of 32%. An isomorphous PtCl(4)(2-) derivative has been obtained.