AIM: The aim of this study was to evaluate the inflammatory response to irrigating solutions injected into the peritoneal cavity of mice. METHODOLOGY: Sixty mice received intra-peritoneal injections of 0.3 mL of 0.5% sodium hypochlorite, 2.0% chlorhexidine digluconate or phosphate buffered saline (PBS, control). Five animals of each group were sacrificed at 4, 24, 48 h and 7 days after the injection. Liquid from the peritoneal cavity of each animal was collected for the total and differential counting of inflammatory cells and protein leakage. RESULTS: The 0.5% sodium hypochlorite solution group had greater migration of neutrophils and mononuclear cells to the peritoneal cavity from 48 to 168 h (P < 0.05). There was a significant increase in protein leakage to the peritoneal cavity after 4 up to 48 h in the 0.5% sodium hypochlorite group compared to the control group. Protein leakage was similar in all groups at 168 h. The 2.0% chlorhexidine group had similar results to the control group at all time periods. CONCLUSIONS: The 0.5% sodium hypochlorite solution induced an inflammatory response, however, the 2.0% chlorhexidine digluconate solution did not induce a significant inflammatory response.
AIM: The aim of this study was to evaluate the inflammatory response to irrigating solutions injected into the peritoneal cavity of mice. METHODOLOGY: Sixty mice received intra-peritoneal injections of 0.3 mL of 0.5% sodium hypochlorite, 2.0% chlorhexidine digluconate or phosphate buffered saline (PBS, control). Five animals of each group were sacrificed at 4, 24, 48 h and 7 days after the injection. Liquid from the peritoneal cavity of each animal was collected for the total and differential counting of inflammatory cells and protein leakage. RESULTS: The 0.5% sodium hypochlorite solution group had greater migration of neutrophils and mononuclear cells to the peritoneal cavity from 48 to 168 h (P < 0.05). There was a significant increase in protein leakage to the peritoneal cavity after 4 up to 48 h in the 0.5% sodium hypochlorite group compared to the control group. Protein leakage was similar in all groups at 168 h. The 2.0% chlorhexidine group had similar results to the control group at all time periods. CONCLUSIONS: The 0.5% sodium hypochlorite solution induced an inflammatory response, however, the 2.0% chlorhexidine digluconate solution did not induce a significant inflammatory response.
Authors: Marsha R Cole; Min Li; Ravirajsinh Jadeja; Bilal El-Zahab; Daniel Hayes; Jeffery A Hobden; Marlene E Janes; Isiah M Warner Journal: J Antimicrob Chemother Date: 2013-02-26 Impact factor: 5.790
Authors: Mohammad Ali Saghiri; Abbas Delvarani; Payman Mehrvarzfar; Mohsen Nikoo; Mehrdad Lotfi; Kasra Karamifar; Kamal Asgar; Sahar Dadvand Journal: Saudi Dent J Date: 2011-03-09
Authors: Tulio G V Gama; Julio C Machado de Oliveira; Ernani C Abad; Isabela N Rôças; José F Siqueira Journal: Clin Oral Investig Date: 2008-04-10 Impact factor: 3.573