OBJECTIVE: To determine whether the activation state of polymorphonuclear neutrophils (PMNs) and monocytes contributes to the inflammatory response after cardiopulmonary bypass (CPB) in pediatric cardiac surgery. DESIGN: Observational prospective clinical study. SETTING: Pediatric intensive care unit of a university hospital. PATIENTS: Twenty pediatric patients before and after open heart surgery with CPB. MEASUREMENTS: Cell counts of circulating PMNs and monocytes as well as phenotypic and functional analysis of these cells, and plasma levels of myeloperoxidase. RESULTS: Levels of myeloperoxidase (a marker of PMN degranulation) were significantly elevated after CPB (2.9+/-1.6 ng/ml before CPB versus 13.7+/-6.5 ng/ml after CPB, p=0.0001). However, PMN function, as measured by surface expression of CD11b/CD18 and phagocytic respiratory burst, was reduced. In contrast, the phagocytic respiratory burst of circulating monocytes was increased in some patients and there was a correlation with the increase of monocyte cell count after CPB (r=0.63, p=0.015). CONCLUSIONS: After the end of CPB, there was an ongoing inflammatory process. In particular, there was an activation of monocytes after the end of CPB.
OBJECTIVE: To determine whether the activation state of polymorphonuclear neutrophils (PMNs) and monocytes contributes to the inflammatory response after cardiopulmonary bypass (CPB) in pediatric cardiac surgery. DESIGN: Observational prospective clinical study. SETTING: Pediatric intensive care unit of a university hospital. PATIENTS: Twenty pediatric patients before and after open heart surgery with CPB. MEASUREMENTS: Cell counts of circulating PMNs and monocytes as well as phenotypic and functional analysis of these cells, and plasma levels of myeloperoxidase. RESULTS: Levels of myeloperoxidase (a marker of PMN degranulation) were significantly elevated after CPB (2.9+/-1.6 ng/ml before CPB versus 13.7+/-6.5 ng/ml after CPB, p=0.0001). However, PMN function, as measured by surface expression of CD11b/CD18 and phagocytic respiratory burst, was reduced. In contrast, the phagocytic respiratory burst of circulating monocytes was increased in some patients and there was a correlation with the increase of monocyte cell count after CPB (r=0.63, p=0.015). CONCLUSIONS: After the end of CPB, there was an ongoing inflammatory process. In particular, there was an activation of monocytes after the end of CPB.
Authors: Gonzalo Sirgo; José Luis Pérez-Vela; Pablo Morales; Manuel Del Rey; Joan Vendrell; Cristina Gutierrez; Jordi Rello Journal: Intensive Care Med Date: 2006-03-01 Impact factor: 17.440
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Authors: Rossitza P Pironkova; Joseph Giamelli; Howard Seiden; Vincent A Parnell; Dorota Gruber; Cristina P Sison; Czeslawa Kowal; Kaie Ojamaa Journal: Exp Ther Med Date: 2017-05-22 Impact factor: 2.447