| Literature DB >> 12444703 |
Carsten Fischer1, Leticia Rodríguez, Eugenio P Patallo, Fredilyn Lipata, Alfredo F Braña, Carmen Méndez, Jose A Salas, Jürgen Rohr.
Abstract
Our explorations of glycosyltransferase ElmGT from Streptomyces olivaceus Tü 2353, which shows an interesting flexibility regarding its sugar donor substrate, were extended toward various previously unexplored sugar co-substrates. The studies revealed that ElmGT, which normally transfers L-rhamnose to 8-demethyltetracenomycin C as a crucial biosynthetic step in elloramycin biosynthesis, is also able to process an activated non-deoxygenated sugar, NDP-D-glucose, as well as NDP-L-digitoxose, which is the first example of an NDP-L-sugar co-substrate of ElmGT possessing an axial 3-OH group. The structures of the resulting novel elloramycin analogues of these experiments, 8-demethyl-8-L-digitoxosyltetracenomycin C (4) and 8-demethyl-8-D-glucosyltetracenomycin C (7), were elucidated mainly by (1)H and (13)C NMR spectroscopy and by mass spectrometry.Entities:
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Year: 2002 PMID： 12444703 DOI： 10.1021/np020112z
Source DB: PubMed Journal: J Nat Prod ISSN： 0163-3864 Impact factor: 4.050