BACKGROUND: This study aimed to evaluate the integrity of anastomotic wound healing after digestive surgery under septic conditions and define the participation of local expression of tumor necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10) around the anastomotic segment. MATERIALS AND METHODS: Experimental animals were divided into lipopolysaccharide (LPS) and control groups, which had either LPS or normal saline solution injected into the peritoneal cavity 24 h before transection and anastomosis of the colon. Anastomotic bursting pressure (ABP) and tissue hydroxyproline concentration (HP) were measured as indicators of wound healing. Immunohistochemical staining for TNF-alpha and IL-10 on tissue samples obtained from the anastomotic segment were examined 1, 6, and 24 h after the operation. The reactive cells were counted under light microscopy. RESULTS: ABP and HP were significantly lower in the LPS group than in the control group 7 days after surgery. In the LPS group, TNF-alpha expression increased about threefold over that in the control group 1 h after the operation. TNF-alpha-reactive cells were observed until 24 h after the operation in the LPS group, but not in the control group. On the other hand, IL-10 was not expressed in the control group during the observed period, whereas IL-10 was observed 24 h after the operation in the LPS group. CONCLUSIONS: It is suggested that anastomotic wound healing was impaired after the digestive surgery in animals treated with intraperitoneal LPS, and that local expression of TNF-alpha and IL-10 at the anastomotic site acts as an inhibitory factor in the wound healing process.
BACKGROUND: This study aimed to evaluate the integrity of anastomotic wound healing after digestive surgery under septic conditions and define the participation of local expression of tumor necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10) around the anastomotic segment. MATERIALS AND METHODS: Experimental animals were divided into lipopolysaccharide (LPS) and control groups, which had either LPS or normal saline solution injected into the peritoneal cavity 24 h before transection and anastomosis of the colon. Anastomotic bursting pressure (ABP) and tissue hydroxyproline concentration (HP) were measured as indicators of wound healing. Immunohistochemical staining for TNF-alpha and IL-10 on tissue samples obtained from the anastomotic segment were examined 1, 6, and 24 h after the operation. The reactive cells were counted under light microscopy. RESULTS: ABP and HP were significantly lower in the LPS group than in the control group 7 days after surgery. In the LPS group, TNF-alpha expression increased about threefold over that in the control group 1 h after the operation. TNF-alpha-reactive cells were observed until 24 h after the operation in the LPS group, but not in the control group. On the other hand, IL-10 was not expressed in the control group during the observed period, whereas IL-10 was observed 24 h after the operation in the LPS group. CONCLUSIONS: It is suggested that anastomotic wound healing was impaired after the digestive surgery in animals treated with intraperitoneal LPS, and that local expression of TNF-alpha and IL-10 at the anastomotic site acts as an inhibitory factor in the wound healing process.
Authors: Magnus S Ågren; Thomas L Andersen; Line Andersen; Christine Bruun Schiødt; Vikas Surve; Troels T Andreassen; Juha Risteli; Lennart E Franzén; Jean-Marie Delaissé; Anne-Marie Heegaard; Lars N Jorgensen Journal: Int J Colorectal Dis Date: 2010-12-31 Impact factor: 2.571
Authors: Dimitrios Pantelis; Anke Beissel; Philip Kahl; Tim O Vilz; Burkhard Stoffels; Sven Wehner; Joerg C Kalff Journal: Int J Colorectal Dis Date: 2011-03-15 Impact factor: 2.571
Authors: Emmeline G Peters; Boudewijn J J Smeets; Marloes Dekkers; Marc D Buise; Wouter J de Jonge; Gerrit D Slooter; Tammo S de Vries Reilingh; Johannes A Wegdam; Grard A P Nieuwenhuijzen; Harm J T Rutten; Ignace H J T de Hingh; Mickael Hiligsmann; Wim A Buurman; Misha D P Luyer Journal: Trials Date: 2015-01-27 Impact factor: 2.279
Authors: Pedro Henrique Alves de Morais; Vinícius Lacerda Ribeiro; Igor Eduardo Caetano de Farias; Luiz Eduardo Almeida Silva; Fabiana Pirani Carneiro; Joel Paulo Russomano Veiga; João Batista de Sousa Journal: World J Emerg Surg Date: 2012-08-22 Impact factor: 5.469