Literature DB >> 12442261

Organic disulfides as a means to generate streak-free two-dimensional maps with narrow range basic immobilized pH gradient strips as first dimension.

Ingmar Olsson1, Kjell Larsson, Ronnie Palmgren, Bengt Bjellqvist.   

Abstract

Streaking is a severe problem when narrow range basic immobilized pH gradient strips are used as the first dimension of two-dimensional (2-D) electrophoresis. It is demonstrated that this cysteinyl related streaking is eliminated when focusing is done in the presence of hydroxyethyl disulfide (DeStreak). Use of DeStreak also results in 2-D maps with simplified spot patterns and improved reproducibility.

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Year:  2002        PMID： 12442261     DOI： 10.1002/1615-9861(200211)2:11<1630::AID-PROT1630>3.0.CO;2-N

Source DB:  PubMed          Journal:  Proteomics        ISSN： 1615-9853            Impact factor:   3.984


  11 in total

1.  A proteomics approach to the study of absorption, distribution, metabolism, excretion, and toxicity.

Authors:  Helena Nordvarg; John Flensburg; Ola Rönn; Johan Ohman; Rita Marouga; Bo Lundgren; Daniel Haid; Eva Malmport; Jan Goscinski; Lena Hörnsten; Michaela Scigelova; Stephanie Bourin; Per Garberg; Gary Woffendin; David Fenyö; Hélène Bergling; Erik Forsberg
Journal:  J Biomol Tech       Date:  2004-12

2.  Optimisation of a 2-D gel electrophoresis protocol for the human-pathogenic fungus Aspergillus fumigatus.

Authors:  Olaf Kniemeyer; Franziska Lessing; Olaf Scheibner; Christian Hertweck; Axel A Brakhage
Journal:  Curr Genet       Date:  2005-12-17       Impact factor: 3.886

3.  Avoiding acidic region streaking in two-dimensional gel electrophoresis: case study with two bacterial whole cell protein extracts.

Authors:  Arnab Roy; Umesh Varshney; Debnath Pal
Journal:  J Biosci       Date:  2014-09       Impact factor: 1.826

4.  Multiple vitellogenins and product yolk proteins in striped bass, Morone saxatilis: molecular characterization and processing during oocyte growth and maturation.

Authors:  V N Williams; B J Reading; N Hiramatsu; H Amano; N Glassbrook; A Hara; C V Sullivan
Journal:  Fish Physiol Biochem       Date:  2013-09-05       Impact factor: 2.794

5.  Improved membrane protein solubilization and clean-up for optimum two-dimensional electrophoresis utilizing GLUT-1 as a classic integral membrane protein.

Authors:  K Devraj; R Geguchadze; M E Klinger; W M Freeman; A Mokashi; R A Hawkins; I A Simpson
Journal:  J Neurosci Methods       Date:  2009-07-23       Impact factor: 2.390

6.  About thiol derivatization and resolution of basic proteins in two-dimensional electrophoresis.

Authors:  Sylvie Luche; Hélène Diemer; Chistophe Tastet; Mireille Chevallet; Alain Van Dorsselaer; Emmanuelle Leize-Wagner; Thierry Rabilloud
Journal:  Proteomics       Date:  2004-03       Impact factor: 3.984

7.  Optimized sample preparation for two-dimensional gel electrophoresis of soluble proteins from chicken bursa of Fabricius.

Authors:  Yongping Wu; Jiyong Zhou; Xin Zhang; Xiaojuan Zheng; Xuetao Jiang; Lixue Shi; Wei Yin; Junhua Wang
Journal:  Proteome Sci       Date:  2009-10-08       Impact factor: 2.480

8.  Technical updates to basic proteins focalization using IPG strips.

Authors:  Jordane Dépagne; François Chevalier
Journal:  Proteome Sci       Date:  2012-09-06       Impact factor: 2.480

9.  Trypanosoma cruzi alkaline 2-DE: Optimization and application to comparative proteome analysis of flagellate life stages.

Authors:  Adriana D Magalhães; Sébastien Charneau; Jaime Paba; Rafael A P Guércio; Antonio R L Teixeira; Jaime M Santana; Marcelo V Sousa; Carlos A O Ricart
Journal:  Proteome Sci       Date:  2008-09-08       Impact factor: 2.480

10.  Technical variability of 2D gel electrophoresis - Application to soybean allergens.

Authors:  Catherine Pallen; Claire Friry-Santini; Corinne Herouet-Guicheney; Annabelle Capt
Journal:  Toxicol Rep       Date:  2014-09-16
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