Literature DB >> 12437977

The substrate specificity of tripartite efflux systems of Pseudomonas aeruginosa is determined by the RND component.

Takeshi Murata1, Misato Kuwagaki, Tomoko Shin, Naomasa Gotoh, Takeshi Nishino.   

Abstract

The tripartite efflux systems MexAB-OprM and MexCD-OprJ of Pseudomonas aeruginosa each display characteristic substrate specificity against a variety of antimicrobial agents. The chimeric efflux system MexC-MexB-OprJ/DeltaMexD constructed by exchange of MexD with MexB endowed the recombinant host the same resistance profile as MexAB-OprM rather than MexCD-OprJ. The change of substrate specificity was shown to be due to extrusion from the chimeric efflux system by cellular accumulation experiments using tetracycline, erythromycin, and ethidium bromide. Thus, we conclude that MexB and MexD are primary components of the efflux system responsible for sorting extrusion substrates.

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Year:  2002        PMID： 12437977     DOI： 10.1016/s0006-291x(02)02626-8

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  8 in total

1.  Contribution of resistance-nodulation-division efflux pump operon smeU1-V-W-U2-X to multidrug resistance of Stenotrophomonas maltophilia.

Authors:  Chao-Hsien Chen; Chiang-Ching Huang; Tsao-Chuen Chung; Rouh-Mei Hu; Yi-Wei Huang; Tsuey-Ching Yang
Journal:  Antimicrob Agents Chemother       Date:  2011-09-19       Impact factor: 5.191

2.  Characterization of a resistance-nodulation-cell division transporter system associated with the syr-syp genomic island of Pseudomonas syringae pv. syringae.

Authors:  Hyojeung Kang; Dennis C Gross
Journal:  Appl Environ Microbiol       Date:  2005-09       Impact factor: 4.792

3.  Substrate competition studies using whole-cell accumulation assays with the major tripartite multidrug efflux pumps of Escherichia coli.

Authors:  Christopher A Elkins; Lisa B Mullis
Journal:  Antimicrob Agents Chemother       Date:  2007-01-08       Impact factor: 5.191

4.  Differential impact of MexB mutations on substrate selectivity of the MexAB-OprM multidrug efflux pump of Pseudomonas aeruginosa.

Authors:  Jocelyn K Middlemiss; Keith Poole
Journal:  J Bacteriol       Date:  2004-03       Impact factor: 3.490

5.  Assembly of the MexAB-OprM multidrug efflux system of Pseudomonas aeruginosa: identification and characterization of mutations in mexA compromising MexA multimerization and interaction with MexB.

Authors:  Dominic Nehme; Xian-Zhi Li; Rachel Elliot; Keith Poole
Journal:  J Bacteriol       Date:  2004-05       Impact factor: 3.490

6.  Evolution of a Heavy Metal Homeostasis/Resistance Island Reflects Increasing Copper Stress in Enterobacteria.

Authors:  Benjamin M Staehlin; John G Gibbons; Antonis Rokas; Thomas V O'Halloran; Jason C Slot
Journal:  Genome Biol Evol       Date:  2016-02-17       Impact factor: 3.416

7.  Molecular Determinants of the Promiscuity of MexB and MexY Multidrug Transporters of Pseudomonas aeruginosa.

Authors:  Venkata K Ramaswamy; Attilio V Vargiu; Giuliano Malloci; Jürg Dreier; Paolo Ruggerone
Journal:  Front Microbiol       Date:  2018-06-01       Impact factor: 5.640

8.  RND type efflux pump system MexAB-OprM of Pseudomonas aeruginosa selects bacterial languages, 3-oxo-acyl-homoserine lactones, for cell-to-cell communication.

Authors:  Shu Minagawa; Hiroyuki Inami; Tomohisa Kato; Shinji Sawada; Tatsuya Yasuki; Shinichi Miyairi; Manabu Horikawa; Jun Okuda; Naomasa Gotoh
Journal:  BMC Microbiol       Date:  2012-05-10       Impact factor: 3.605
  8 in total

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