AIMS/HYPOTHESIS: We carried out global transcript profiling to identify differentially expressed skeletal muscle genes in insulin resistance, a major risk factor for Type II (non-insulin-dependent) diabetes mellitus. This approach also complemented the ongoing genomic linkage analyses to identify genes linked to insulin resistance and diabetes in Pima Indians. METHODS: We compared gene expression profiles of skeletal muscle tissues from 18 insulin-sensitive versus 17 insulin-resistant equally obese, non-diabetic Pima Indians using oligonucleotide arrays consisting of about 40,600 transcripts of known genes and expressed sequence tags, and analysed the results with the Wilcoxon rank sum test. We verified the mRNA expression of ten differentially (best-ranked) and ten similarly (worst-ranked) genes using quantitative Real Time PCR. RESULTS: There were 185 differentially expressed transcripts by the rank sum test. The differential expressions of two out of the ten best-ranked genes were confirmed and the similar expressions of all ten worst-ranked genes were reproduced. CONCLUSION/ INTERPRETATION: Of the 185 differentially expressed transcripts, 20 per cent were true positives and some could generate new hypotheses about the aetiology or pathophysiology of insulin resistance. Furthermore, differentially expressed genes in chromosomal regions with linkage to diabetes and insulin resistance serve as new diabetes susceptibility genes.
AIMS/HYPOTHESIS: We carried out global transcript profiling to identify differentially expressed skeletal muscle genes in insulin resistance, a major risk factor for Type II (non-insulin-dependent) diabetes mellitus. This approach also complemented the ongoing genomic linkage analyses to identify genes linked to insulin resistance and diabetes in Pima Indians. METHODS: We compared gene expression profiles of skeletal muscle tissues from 18 insulin-sensitive versus 17 insulin-resistant equally obese, non-diabetic Pima Indians using oligonucleotide arrays consisting of about 40,600 transcripts of known genes and expressed sequence tags, and analysed the results with the Wilcoxon rank sum test. We verified the mRNA expression of ten differentially (best-ranked) and ten similarly (worst-ranked) genes using quantitative Real Time PCR. RESULTS: There were 185 differentially expressed transcripts by the rank sum test. The differential expressions of two out of the ten best-ranked genes were confirmed and the similar expressions of all ten worst-ranked genes were reproduced. CONCLUSION/ INTERPRETATION: Of the 185 differentially expressed transcripts, 20 per cent were true positives and some could generate new hypotheses about the aetiology or pathophysiology of insulin resistance. Furthermore, differentially expressed genes in chromosomal regions with linkage to diabetes and insulin resistance serve as new diabetes susceptibility genes.
Authors: Laura Formentini; Alexander J Ryan; Manuel Gálvez-Santisteban; Leslie Carter; Pam Taub; John D Lapek; David J Gonzalez; Francisco Villarreal; Theodore P Ciaraldi; José M Cuezva; Robert R Henry Journal: Diabetologia Date: 2017-08-02 Impact factor: 10.122
Authors: Paul K Tan; Thomas J Downey; Edward L Spitznagel; Pin Xu; Dadin Fu; Dimiter S Dimitrov; Richard A Lempicki; Bruce M Raaka; Margaret C Cam Journal: Nucleic Acids Res Date: 2003-10-01 Impact factor: 16.971
Authors: Alex Rajna; Heather Gibling; Ousseynou Sarr; Sarthak Matravadia; Graham P Holloway; David M Mutch Journal: Physiol Genomics Date: 2018-05-04 Impact factor: 3.107