Literature DB >> 12432341

Quantifying DNA concentrations using fluorometry: a comparison of fluorophores.

Kalpana Rengarajan1, Stphen M Cristol, Milan Mehta, John M Nickerson.   

Abstract

Detection of low concentrations of DNA is important in vision research because many animal models only provide scant samples of ocular tissue. Quantitative analysis of low concentrations of double stranded DNA is now feasible using fluorometry with newer fluorophores. This technique offers a rapid way to evaluate the DNA content of samples based on the measurement of fluorescence enhancement emitted by fluorophore-bound DNA and is more sensitive than absorption spectrometry. The purpose of this study was to compare the sensitivity of several different fluorophores for measuring DNA concentrations by fluorometry. Based on our studies, we conclude that SYBR Green I and PicoGreen are substantially more sensitive for quantifying DNA concentrations than ethidium bromide and Hoechst 33258.

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Year:  2002        PMID： 12432341

Source DB:  PubMed          Journal:  Mol Vis        ISSN： 1090-0535            Impact factor:   2.367


  18 in total

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4.  Comparison of commercial DNA extraction kits for extraction of bacterial genomic DNA from whole-blood samples.

Authors:  K Smith; M A Diggle; S C Clarke
Journal:  J Clin Microbiol       Date:  2003-06       Impact factor: 5.948

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Journal:  Cytotechnology       Date:  2011-05-12       Impact factor: 2.058

7.  Visual automated fluorescence electrophoresis provides simultaneous quality, quantity, and molecular weight spectra for genomic DNA from archived neonatal blood spots.

Authors:  Tara L Klassen; Janice Drabek; Torjbörn Tomson; Olafur Sveinsson; Ulrika von Döbeln; Jeffrey L Noebels; Alicia M Goldman
Journal:  J Mol Diagn       Date:  2013-03-19       Impact factor: 5.568

8.  Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications.

Authors:  Hubert Zipper; Herwig Brunner; Jürgen Bernhagen; Frank Vitzthum
Journal:  Nucleic Acids Res       Date:  2004-07-12       Impact factor: 16.971

9.  Simple and inexpensive fluorescence-based technique for high-throughput antimalarial drug screening.

Authors:  Martin Smilkstein; Nongluk Sriwilaijaroen; Jane Xu Kelly; Prapon Wilairat; Michael Riscoe
Journal:  Antimicrob Agents Chemother       Date:  2004-05       Impact factor: 5.191

10.  Binding fullerenol C(60)(OH)(24) to dsDNA.

Authors:  Mariana Pinteala; Andrei Dascalu; Cezar Ungurenasu
Journal:  Int J Nanomedicine       Date:  2009-09-10
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