Literature DB >> 12429922

Improved flow cytometric analysis of the budding yeast cell cycle.

Steven B Haase1, Steven I Reed.   

Abstract

The budding yeast, Saccharomyces cerevisiae has been a remarkably useful model system for the study of eukaryotic cell cycle regulation. Flow cytometric analysis of DNA content in budding yeast has become a standard tool for the analysis of cell cycle progression. However, popular protocols utilizing the DNA binding dye, propidium iodide, suffer from a number of drawbacks that confound accurate analysis by flow cytometry. Here we show the utility of the DNA binding dye, SYTOX Green, in the cell cycle analysis of yeast. Samples analyzed using SYTOX Green exhibited better coefficients of variation, improved linearity between DNA content and fluorescence, and decreased peak drift associated with changes in dye concentration, growth conditions or cell size.

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Year:  2002        PMID： 12429922

Source DB:  PubMed          Journal:  Cell Cycle        ISSN： 1551-4005            Impact factor:   4.534


  105 in total

1.  S-phase cyclin-dependent kinases promote sister chromatid cohesion in budding yeast.

Authors:  W-S Hsu; S L Erickson; H-J Tsai; C A Andrews; A C Vas; D J Clarke
Journal:  Mol Cell Biol       Date:  2011-04-25       Impact factor: 4.272

2.  Promoter strength influences the S phase requirement for establishment of silencing at the Saccharomyces cerevisiae silent mating type Loci.

Authors:  Jie Ren; Chia-Lin Wang; Rolf Sternglanz
Journal:  Genetics       Date:  2010-08-02       Impact factor: 4.562

3.  Delineation of WRN helicase function with EXO1 in the replicational stress response.

Authors:  Monika Aggarwal; Joshua A Sommers; Christa Morris; Robert M Brosh
Journal:  DNA Repair (Amst)       Date:  2010-05-05

4.  Cdc7-Dbf4 phosphorylates MCM proteins via a docking site-mediated mechanism to promote S phase progression.

Authors:  Yi-Jun Sheu; Bruce Stillman
Journal:  Mol Cell       Date:  2006-10-06       Impact factor: 17.970

5.  Constitutively high dNTP concentration inhibits cell cycle progression and the DNA damage checkpoint in yeast Saccharomyces cerevisiae.

Authors:  Andrei Chabes; Bruce Stillman
Journal:  Proc Natl Acad Sci U S A       Date:  2007-01-16       Impact factor: 11.205

6.  Inhibition of Toxoplasma gondii growth by pyrrolidine dithiocarbamate is cell cycle specific and leads to population synchronization.

Authors:  Magnolia M Conde de Felipe; Margaret M Lehmann; Maria E Jerome; Michael W White
Journal:  Mol Biochem Parasitol       Date:  2007-09-21       Impact factor: 1.759

7.  A systematic screen for transcriptional regulators of the yeast cell cycle.

Authors:  Michael A White; Linda Riles; Barak A Cohen
Journal:  Genetics       Date:  2008-11-24       Impact factor: 4.562

8.  A mitotic topoisomerase II checkpoint in budding yeast is required for genome stability but acts independently of Pds1/securin.

Authors:  Catherine A Andrews; Amit C Vas; Brian Meier; Juan F Giménez-Abián; Laura A Díaz-Martínez; Julie Green; Stacy L Erickson; Kristyn E Vanderwaal; Wei-Shan Hsu; Duncan J Clarke
Journal:  Genes Dev       Date:  2006-05-01       Impact factor: 11.361

9.  Physiological and transcriptional responses of anaerobic chemostat cultures of Saccharomyces cerevisiae subjected to diurnal temperature cycles.

Authors:  Marit Hebly; Dick de Ridder; Erik A F de Hulster; Pilar de la Torre Cortes; Jack T Pronk; Pascale Daran-Lapujade
Journal:  Appl Environ Microbiol       Date:  2014-05-09       Impact factor: 4.792

10.  A conserved deubiquitinating enzyme controls cell growth by regulating RNA polymerase I stability.

Authors:  Lauren A Richardson; Benjamin J Reed; J Michael Charette; Emily F Freed; Eric K Fredrickson; Melissa N Locke; Susan J Baserga; Richard G Gardner
Journal:  Cell Rep       Date:  2012-08-16       Impact factor: 9.423
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