BACKGROUND: Ischemia/reperfusion (I/R) injury induces an inflammatory response and production of reactive oxygen species (ROS), which affects the organs remote to the sites of I/R. The aim was to assess the hepatic changes after renal I/R injury. MATERIALS AND METHODS: Twenty mice were subjected to either sham operation or varying degrees of renal I/R injury. Hepatic TNF-alpha levels, myeloperoxidase (MPO), superoxide dismutase (SOD), and catalase (CAT) activities and reduced glutathione (GSH) levels, thiobarbituric acid-reactive substances (TBARS), and protein carbonyl levels were evaluated to show hepatic response to renal I/R injury. RESULTS: Hepatic tumor necrosis factor-alpha levels were found to be increased significantly after 30 min ischemia-1 h reperfusion and remained elevated through 60 min ischemia-1 h reperfusion. Supporting the neutrophil recruitment, about 10-fold increase in MPO activity was detected after 30 min ischemia-1 h reperfusion. Antioxidant enzymes were detected to be decreased after 30 min ischemia-1 h reperfusion and reached to the minimum levels after 60 min ischemia-1 h reperfusion. Decreased levels of GSH and increased levels of TBARS and protein carbonyls after 60 min ischemia-1 h reperfusion supported the ROS-mediated biomolecular alterations. CONCLUSIONS: A minumum of 30 min ischemia-1 h reperfusion is enough to elicit remote effects of renal I/R injury. Care should be taken to protect other organs remote from I/R sites especially during renal surgery.
BACKGROUND:Ischemia/reperfusion (I/R) injury induces an inflammatory response and production of reactive oxygen species (ROS), which affects the organs remote to the sites of I/R. The aim was to assess the hepatic changes after renal I/R injury. MATERIALS AND METHODS: Twenty mice were subjected to either sham operation or varying degrees of renal I/R injury. Hepatic TNF-alpha levels, myeloperoxidase (MPO), superoxide dismutase (SOD), and catalase (CAT) activities and reduced glutathione (GSH) levels, thiobarbituric acid-reactive substances (TBARS), and protein carbonyl levels were evaluated to show hepatic response to renal I/R injury. RESULTS: Hepatic tumor necrosis factor-alpha levels were found to be increased significantly after 30 min ischemia-1 h reperfusion and remained elevated through 60 min ischemia-1 h reperfusion. Supporting the neutrophil recruitment, about 10-fold increase in MPO activity was detected after 30 min ischemia-1 h reperfusion. Antioxidant enzymes were detected to be decreased after 30 min ischemia-1 h reperfusion and reached to the minimum levels after 60 min ischemia-1 h reperfusion. Decreased levels of GSH and increased levels of TBARS and protein carbonyls after 60 min ischemia-1 h reperfusion supported the ROS-mediated biomolecular alterations. CONCLUSIONS: A minumum of 30 min ischemia-1 h reperfusion is enough to elicit remote effects of renal I/R injury. Care should be taken to protect other organs remote from I/R sites especially during renal surgery.
Authors: Cemal Goktas; Abdurrahman Coskun; Zerrin Bicik; Rahim Horuz; Ibrahim Unsal; Mustafa Serteser; Selami Albayrak; Kemal Sarıca Journal: Urol Res Date: 2012-10
Authors: Laura E White; Rachel J Santora; Yan Cui; Frederick A Moore; Heitham T Hassoun Journal: Am J Physiol Lung Cell Mol Physiol Date: 2012-06-22 Impact factor: 5.464
Authors: Sang Won Park; Sean W C Chen; Mihwa Kim; Kevin M Brown; Jay K Kolls; Vivette D D'Agati; H Thomas Lee Journal: Lab Invest Date: 2010-08-09 Impact factor: 5.662
Authors: Heitham T Hassoun; Mihaela L Lie; Dmitry N Grigoryev; Manchang Liu; Rubin M Tuder; Hamid Rabb Journal: Am J Physiol Renal Physiol Date: 2009-04-29
Authors: Sang Won Park; Mihwa Kim; Joo Yun Kim; Ahrom Ham; Kevin M Brown; Yuko Mori-Akiyama; André J Ouellette; Vivette D D'Agati; H Thomas Lee Journal: J Immunol Date: 2012-10-29 Impact factor: 5.422