Qian Xiang1, Shou-Yi Yu, Hong Wang. 1. Department of Medical Teaching and Research, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Abstract
OBJECTIVE: To study the resistance mechanism of quinolone against Shigellae flexneri. METHODS: The N-terminal coding region of gyrA gene of 38 quinolone-resistant Shigellae flexneri were amplified by PCR and mutations detected by single- strand conformation polymorphism (SSCP), followed by sequence analysis. RESULTS: Eight isolates were found to be positive for gyrA mutations, and DNA sequence analysis of gyrA gene revealed 2 mutations that resulted in changes of the amino acids (Ser-83--Leu, Asp-87--Gly). CONCLUSION: gryA gene mutations is closely related to high resistance of Shigellae flexneri to quinolone.
OBJECTIVE: To study the resistance mechanism of quinolone against Shigellae flexneri. METHODS: The N-terminal coding region of gyrA gene of 38 quinolone-resistant Shigellae flexneri were amplified by PCR and mutations detected by single- strand conformation polymorphism (SSCP), followed by sequence analysis. RESULTS: Eight isolates were found to be positive for gyrA mutations, and DNA sequence analysis of gyrA gene revealed 2 mutations that resulted in changes of the amino acids (Ser-83--Leu, Asp-87--Gly). CONCLUSION: gryA gene mutations is closely related to high resistance of Shigellae flexneri to quinolone.