OBJECTIVE: To investigate the changes of cytokeratin 19 during oral carcinogenesis. METHODS: 53 specimens including normal oral mucosa, oral epithelial hyperplasia, oral epithelial dysplasia and oral squamous cell carcinoma were investigated by immunohistochemistry, SDS-PAGE and Western blotting. RESULTS: CK19 was detectable in suprabasal cell layers in epithelial dysplasia and in oral cancer, especially in poor-differentiated cancerous cells. With the lesions getting worse, the positive rate, the intensity and the constituent ratio of CK19 raised significantly. CONCLUSIONS: The results suggest that the CK19 expression in suprabasal cell layers of oral mucosa can be used as a marker of diagnosis of oral precancerous lesions and CK19 expression is the initial events during oral carcinogenesis.
OBJECTIVE: To investigate the changes of cytokeratin 19 during oral carcinogenesis. METHODS: 53 specimens including normal oral mucosa, oral epithelial hyperplasia, oral epithelial dysplasia and oral squamous cell carcinoma were investigated by immunohistochemistry, SDS-PAGE and Western blotting. RESULTS:CK19 was detectable in suprabasal cell layers in epithelial dysplasia and in oral cancer, especially in poor-differentiated cancerous cells. With the lesions getting worse, the positive rate, the intensity and the constituent ratio of CK19 raised significantly. CONCLUSIONS: The results suggest that the CK19 expression in suprabasal cell layers of oral mucosa can be used as a marker of diagnosis of oral precancerous lesions and CK19 expression is the initial events during oral carcinogenesis.
Authors: Ali Yousif Babiker; Arshad H Rahmani; Mohamed S Abdalaziz; Aqel Albutti; Salah M Aly; Hussain Gadelkareem Ahmed Journal: Int J Clin Exp Med Date: 2014-06-15