Literature DB >> 12418785

Improved immunobinding test using monoclonal antibodies for detection of Mycoplasma bovis in milk.

F Infante1, F Infante1, G H Flores-Gutiérrez.   

Abstract

The aim of this study was to evaluate an improved immunobinding test (IBT) using monoclonal antibodies to identify Mycoplasma bovis in naturally infected milk. The IBT and the improved IBT were highly specific and had an immunologic sensitivity of 5 x 10(3) colony-forming units per milliliter. The results for the 2 methods agreed in the 130 milk samples tested. However, the IBT required 158 min, whereas the improved IBT required only 110 min. In addition, the improved IBT used smaller quantities of antibodies and conjugates.

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Year:  2002        PMID： 12418785      PMCID： PMC227017     

Source DB:  PubMed          Journal:  Can J Vet Res        ISSN： 0830-9000            Impact factor:   1.310


  7 in total

1.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

2.  Immunobinding assay for detection of Mycoplasma bovis in milk.

Authors:  F Infante Martinez; D E Jasper; J L Stott; J S Cullor; J D Dellinger
Journal:  Can J Vet Res       Date:  1990-04       Impact factor: 1.310

3.  Detecting Mycoplasma bovis in milk by enzyme-linked immunosorbent assay, using monoclonal antibodies.

Authors:  J T Boothby; R Mueller; D E Jasper; C B Thomas
Journal:  Am J Vet Res       Date:  1986-05       Impact factor: 1.156

4.  The role of Mycoplasma in bovine mastitis.

Authors:  D E Jasper
Journal:  J Am Vet Med Assoc       Date:  1982-07-15       Impact factor: 1.936

5.  Detection of Mycoplasma bovis and Mycoplasma agalactiae by oligonucleotide probes complementary to 16S rRNA.

Authors:  J G Mattsson; H Gersdorf; U B Göbel; K E Johansson
Journal:  Mol Cell Probes       Date:  1991-02       Impact factor: 2.365

6.  Agar block technique for identification of mycoplasmas by use of fluorescent antibody.

Authors:  E J Baas; D E Jasper
Journal:  Appl Microbiol       Date:  1972-06

7.  In vitro amplification of the 16S rRNA genes from Mycoplasma bovis and Mycoplasma agalactiae by PCR.

Authors:  Y R Chávez González; C Ros Bascuñana; G Bölske; J G Mattsson; C Fernández Molina; K E Johansson
Journal:  Vet Microbiol       Date:  1995-11       Impact factor: 3.293
  7 in total
  3 in total

1.  Identification and serological specificity of a polysaccharide component from Mycoplasma bovis.

Authors:  B W Brooks; C L Lutze-Wallace; P Lu; R H Robertson
Journal:  Vet Res Commun       Date:  2004-04       Impact factor: 2.459

2.  Use of an immunobinding test on nitrocellulose paper to diagnose caprine brucellosis.

Authors:  E Santellano-Estrada; F Infante; E Díaz-Aparicio; G H Flores-Gutiérrez
Journal:  Vet Res Commun       Date:  2004-01       Impact factor: 2.459

3.  Development of an immunobinding assay with monoclonal antibodies to diagnose Mycoplasma bovis in semen.

Authors:  G H Flores-Gutiérrez; F Infante; J A Salinas-Meléndez; C B Thomas; P C Estrada-Bellmann; F Briones-Encinia
Journal:  Vet Res Commun       Date:  2004-11       Impact factor: 2.459
  3 in total

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