Literature DB >> 12417337

Pyrococcus furiosus ferredoxin is a functional dimer.

M N Hasan1, P L Hagedoorn, W R Hagen.   

Abstract

Pyrococcus furiosus ferredoxin is subject to a monomer/dimer equilibrium as a function of ionic strength. At physiological ionic strength, approximately 0.35 M NaCl, the protein is very predominantly homodimer. The monomeric form exhibits impaired electron transfer on glassy carbon; it also has a decreased S=3/2 over S=1/2 ratio as shown by electron paramagnetic resonance spectroscopy. Even following sterilization at 121 degrees C the dimer is stable in denaturing gel electrophoresis.

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Year:  2002        PMID： 12417337     DOI： 10.1016/s0014-5793(02)03554-8

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  5 in total

1.  Pyrococcus furiosus 4Fe-ferredoxin, chemisorbed on gold, exhibits gated reduction and ionic strength dependent dimerization.

Authors:  M Nahid Hasan; Cees Kwakernaak; Willem G Sloof; Wilfred R Hagen; Hendrik A Heering
Journal:  J Biol Inorg Chem       Date:  2006-05-30       Impact factor: 3.358

2.  Crystal structures of the all-cysteinyl-coordinated D14C variant of Pyrococcus furiosus ferredoxin: [4Fe-4S] ↔ [3Fe-4S] cluster conversion.

Authors:  Monika Nøhr Løvgreen; Maja Martic; Michael S Windahl; Hans E M Christensen; Pernille Harris
Journal:  J Biol Inorg Chem       Date:  2011-04-12       Impact factor: 3.358

3.  Heterometallic [AgFe(3)S (4)] ferredoxin variants: synthesis, characterization, and the first crystal structure of an engineered heterometallic iron-sulfur protein.

Authors:  Maja Martic; Ida Noémi Jakab-Simon; Lærke Tvedebrink Haahr; Wilfred Raymond Hagen; Hans Erik Mølager Christensen
Journal:  J Biol Inorg Chem       Date:  2013-01-08       Impact factor: 3.358

4.  A steady-state and pre-steady-state kinetics study of the tungstoenzyme formaldehyde ferredoxin oxidoreductase from Pyrococcus furiosus.

Authors:  Emile Bol; Nicolette J Broers; Wilfred R Hagen
Journal:  J Biol Inorg Chem       Date:  2007-09-25       Impact factor: 3.358

5.  Physical and functional interplay between PCNA DNA clamp and Mre11-Rad50 complex from the archaeon Pyrococcus furiosus.

Authors:  Gaëlle Hogrel; Yang Lu; Sébastien Laurent; Etienne Henry; Clarisse Etienne; Duy Khanh Phung; Rémi Dulermo; Audrey Bossé; Pierre-François Pluchon; Béatrice Clouet-d'Orval; Didier Flament
Journal:  Nucleic Acids Res       Date:  2018-06-20       Impact factor: 16.971
  5 in total

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