David B Seifer1, Vimaris DeJesus, Karen Hubbard. 1. Department of Obstetrics, Gynecology and Reproductive Sciences, Division of Reproductive Endocrinology and Infertility, UMDNJ-Robert Wood Johnson Medical School, New Brunswick, New Jersey 08901, USA. seiferdb@umdnj.edu
Abstract
OBJECTIVE: To test the hypothesis that mitochondrial DNA (mtDNA) deletions are more prevalent in granulosa cells from women of advanced reproductive age than from younger women undergoing IVF. DESIGN: Granulosa cells screened for presence or absence of the 4977-bp deletion in human mtDNA. SETTING: University-based fertility clinic. PATIENT(S): Twenty-four women divided equally between two groups: </=34 years old and >/=38 years old. INTERVENTION(S): Patients were given gonadotropin stimulation in preparation for IVF with granulosa cells isolated at the time of follicular aspiration. MAIN OUTCOME MEASURE(S): Presence or absence of the 4977-bp deletion in human mtDNA. RESULT(S): Seven out of 12 women analyzed who were <38 years old and 0 out of 12 women who were >38 years old had normal mtDNA as indicated by the presence of the 4977-bp fragment. CONCLUSION(S): These data suggest that women over the age of 38 have granulosa cells that contain a substantial decrease in the level of normal mitochondria as compared with women </=34 years.
OBJECTIVE: To test the hypothesis that mitochondrial DNA (mtDNA) deletions are more prevalent in granulosa cells from women of advanced reproductive age than from younger women undergoing IVF. DESIGN: Granulosa cells screened for presence or absence of the 4977-bp deletion in human mtDNA. SETTING: University-based fertility clinic. PATIENT(S): Twenty-four women divided equally between two groups: </=34 years old and >/=38 years old. INTERVENTION(S): Patients were given gonadotropin stimulation in preparation for IVF with granulosa cells isolated at the time of follicular aspiration. MAIN OUTCOME MEASURE(S): Presence or absence of the 4977-bp deletion in human mtDNA. RESULT(S): Seven out of 12 women analyzed who were <38 years old and 0 out of 12 women who were >38 years old had normal mtDNA as indicated by the presence of the 4977-bp fragment. CONCLUSION(S): These data suggest that women over the age of 38 have granulosa cells that contain a substantial decrease in the level of normal mitochondria as compared with women </=34 years.
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