OBJECTIVE: To study the distribution of hemorrhagic fever with renal syndrome virus (HFRSV) in mites. METHODS: In situ reverse transcription-polymerase chain reaction (IS RT-PCR) was used for detecting the distribution of HFRSV in mites. RESULTS: HFRSV RNA was mainly located in ovary and mid-gut tissues of gamasid mites and chigger mites. The positive signal intensity in the third and fourth generations of gamasid mite was stronger than that in the first and second generations, and that in nymph of chigger mite more than larva. CONCLUSION: Both chigger mite and gamasid mite could play an important role in the transmission of HFRSV.
OBJECTIVE: To study the distribution of hemorrhagic fever with renal syndrome virus (HFRSV) in mites. METHODS: In situ reverse transcription-polymerase chain reaction (IS RT-PCR) was used for detecting the distribution of HFRSV in mites. RESULTS: HFRSV RNA was mainly located in ovary and mid-gut tissues of gamasid mites and chigger mites. The positive signal intensity in the third and fourth generations of gamasid mite was stronger than that in the first and second generations, and that in nymph of chigger mite more than larva. CONCLUSION: Both chigger mite and gamasid mite could play an important role in the transmission of HFRSV.