Literature DB >> 12409432

PCR-based detection of Bacillus anthracis in formalin-fixed tissue from a patient receiving ciprofloxacin.

Steven M Levine1, Guillermo Perez-Perez, Asalia Olivares, Herman Yee, Bruce A Hanna, Martin J Blaser.   

Abstract

We demonstrate that Bacillus anthracis may be detected from a formalin-fixed, paraffin-embedded biopsy specimen, even after the patient has received antibiotic treatment. Although traditional PCR methods may not be sufficiently sensitive for anthrax detection in such patients, cycle numbers can be increased or PCR can be repeated by using an aliquot from a previous PCR as the template.

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Year:  2002        PMID： 12409432      PMCID： PMC139675          DOI： 10.1128/JCM.40.11.4360-4362.2002

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  17 in total

1.  Optimisation of DNA and RNA extraction from archival formalin-fixed tissue.

Authors:  N J Coombs; A C Gough; J N Primrose
Journal:  Nucleic Acids Res       Date:  1999-08-15       Impact factor: 16.971

2.  Cutaneous Bacillus anthracis infection.

Authors:  T C Gallagher; B E Strober
Journal:  N Engl J Med       Date:  2001-11-08       Impact factor: 91.245

3.  Index case of fatal inhalational anthrax due to bioterrorism in the United States.

Authors:  L M Bush; B H Abrams; A Beall; C C Johnson
Journal:  N Engl J Med       Date:  2001-11-08       Impact factor: 91.245

4.  Update: Investigation of bioterrorism-related anthrax, 2001.

Authors: 
Journal:  MMWR Morb Mortal Wkly Rep       Date:  2001-11-16       Impact factor: 17.586

Review 5.  Anthrax.

Authors:  M Mock; A Fouet
Journal:  Annu Rev Microbiol       Date:  2001       Impact factor: 15.500

Review 6.  Anthrax as a biological weapon: medical and public health management. Working Group on Civilian Biodefense.

Authors:  T V Inglesby; D A Henderson; J G Bartlett; M S Ascher; E Eitzen; A M Friedlander; J Hauer; J McDade; M T Osterholm; T O'Toole; G Parker; T M Perl; P K Russell; K Tonat
Journal:  JAMA       Date:  1999-05-12       Impact factor: 56.272

7.  The native virulence plasmid combination affects the segregational stability of a theta-replicating shuttle vector in Bacillus anthracis var. New Hampshire.

Authors:  J E Bowen; C P Quinn
Journal:  J Appl Microbiol       Date:  1999-08       Impact factor: 3.772

Review 8.  Application of rapid-cycle real-time polymerase chain reaction for the detection of microbial pathogens: the Mayo-Roche Rapid Anthrax Test.

Authors:  James R Uhl; Constance A Bell; Lynne M Sloan; Mark J Espy; Thomas F Smith; Jon E Rosenblatt; Franklin R Cockerill
Journal:  Mayo Clin Proc       Date:  2002-07       Impact factor: 7.616

9.  Evidence for plasmid-mediated toxin production in Bacillus anthracis.

Authors:  P Mikesell; B E Ivins; J D Ristroph; T M Dreier
Journal:  Infect Immun       Date:  1983-01       Impact factor: 3.441

10.  Bioterrorism-related inhalational anthrax: the first 10 cases reported in the United States.

Authors:  J A Jernigan; D S Stephens; D A Ashford; C Omenaca; M S Topiel; M Galbraith; M Tapper; T L Fisk; S Zaki; T Popovic; R F Meyer; C P Quinn; S A Harper; S K Fridkin; J J Sejvar; C W Shepard; M McConnell; J Guarner; W J Shieh; J M Malecki; J L Gerberding; J M Hughes; B A Perkins
Journal:  Emerg Infect Dis       Date:  2001 Nov-Dec       Impact factor: 6.883
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  1 in total

Review 1.  Exposing a killer: pathologists angle for anthrax.

Authors:  Stephen W Chensue
Journal:  Am J Pathol       Date:  2003-11       Impact factor: 4.307
  1 in total

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