Literature DB >> 12409419

Serological differentiation of infections with dengue virus serotypes 1 to 4 by using recombinant antigens.

Diana Ludolfs1, Stefan Schilling, Jan Altenschmidt, Herbert Schmitz.   

Abstract

The B domains of dengue virus serotypes 1 to 4 were expressed in Escherichia coli. The purified proteins were applied to immunoblot strips to detect serotype-specific antibodies in paired serum samples from 41 patients with primary and secondary dengue infections. A close correlation between the results obtained with the immunoblot strips and by type-specific reverse transcription-PCR (T. Laue, P. Emmerich, and H. Schmitz, J. Clin. Microbiol. 37:2543-2547, 1999) was observed.

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Year:  2002        PMID： 12409419      PMCID： PMC139635          DOI： 10.1128/JCM.40.11.4317-4320.2002

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  20 in total

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3.  Evaluation of recombinant dengue viral envelope B domain protein antigens for the detection of dengue complex-specific antibodies.

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5.  Extensive nucleotide changes and deletions within the envelope glycoprotein gene of Euro-African West Nile viruses.

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7.  Evaluation of envelope domain III-based single chimeric tetravalent antigen and monovalent antigen mixtures for the detection of anti-dengue antibodies in human sera.

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