Literature DB >> 12409413

Direct detection and identification of African trypanosomes by fluorescence in situ hybridization with peptide nucleic acid probes.

M Radwanska1, S Magez, H Perry-O'Keefe, H Stender, J Coull, J M Sternberg, P Büscher, J J Hyldig-Nielsen.   

Abstract

We have developed a rapid and easy to perform fluorescence in situ hybridization test that allows specific identification of trypanosomes from the subgenus Trypanozoon, using peptide nucleic acid probes. Probes were designed to target subgenus-specific sequences on the multiple-copy 18S rRNA, greatly facilitating the detection of a single trypanosome.

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Year:  2002        PMID： 12409413      PMCID： PMC139669          DOI： 10.1128/JCM.40.11.4295-4297.2002

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  18 in total

1.  Detection of trypanosomes in suspected sleeping sickness patients in Uganda using the polymerase chain reaction.

Authors:  J W Kyambadde; J C Enyaru; E Matovu; M Odiit; J F Carasco
Journal:  Bull World Health Organ       Date:  2000       Impact factor: 9.408

2.  Comparative analysis of antibody responses against HSP60, invariant surface glycoprotein 70, and variant surface glycoprotein reveals a complex antigen-specific pattern of immunoglobulin isotype switching during infection by Trypanosoma brucei.

Authors:  M Radwanska; S Magez; A Michel; B Stijlemans; M Geuskens; E Pays
Journal:  Infect Immun       Date:  2000-02       Impact factor: 3.441

Review 3.  Genetic controls for the expression of surface antigens in African trypanosomes.

Authors:  E Pays; L Vanhamme; M Berberof
Journal:  Annu Rev Microbiol       Date:  1994       Impact factor: 15.500

4.  Parasitological detection of Trypanosoma brucei gambiense in serologically negative sleeping-sickness suspects from north-western Uganda.

Authors:  J C Enyaru; E Matovu; M Akol; C Sebikali; J Kyambadde; C Schmidt; R Brun; R Kaminsky; L M Ogwal; F Kansiime
Journal:  Ann Trop Med Parasitol       Date:  1998-12

5.  A card-agglutination test with stained trypanosomes (C.A.T.T.) for the serological diagnosis of T. B. gambiense trypanosomiasis.

Authors:  E Magnus; T Vervoort; N Van Meirvenne
Journal:  Ann Soc Belg Med Trop       Date:  1978

6.  Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose.

Authors:  S M Lanham; D G Godfrey
Journal:  Exp Parasitol       Date:  1970-12       Impact factor: 2.011

7.  Fluorescence In situ hybridization assay using peptide nucleic acid probes for differentiation between tuberculous and nontuberculous mycobacterium species in smears of mycobacterium cultures.

Authors:  H Stender; K Lund; K H Petersen; O F Rasmussen; P Hongmanee; H Miörner; S E Godtfredsen
Journal:  J Clin Microbiol       Date:  1999-09       Impact factor: 5.948

8.  Diagnosis of human trypanosomiasis, due to Trypanosoma brucei gambiense in central Africa, by the polymerase chain reaction.

Authors:  L Penchenier; G Simo; P Grébaut; S Nkinin; C Laveissière; S Herder
Journal:  Trans R Soc Trop Med Hyg       Date:  2000 Jul-Aug       Impact factor: 2.184

9.  Detection of Trypanosoma brucei gambiense, in serologically positive but aparasitaemic sleeping-sickness suspects in Cameroon, by PCR.

Authors:  G D Kanmogne; T Asonganyi; W C Gibson
Journal:  Ann Trop Med Parasitol       Date:  1996-10

10.  Will the real Trypanosoma b. gambiense please stand up.

Authors:  W C Gibson
Journal:  Parasitol Today       Date:  1986-09
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  5 in total

1.  Diagnosing Cutaneous leishmaniasis using Fluorescence in Situ Hybridization: the Sri Lankan Perspective.

Authors:  Thilini Dilhara Jayasena Kaluarachchi; Manjula Manoji Weerasekera; Andrew J McBain; Shalindra Ranasinghe; Renu Wickremasinghe; Surangi Yasawardene; Nisal Jayanetti; Rajitha Wickremasinghe
Journal:  Pathog Glob Health       Date:  2019-08-20       Impact factor: 2.894

Review 2.  Fluorescence In Situ Hybridization (FISH) Tests for Identifying Protozoan and Bacterial Pathogens in Infectious Diseases.

Authors:  Jyotsna S Shah; Ranjan Ramasamy
Journal:  Diagnostics (Basel)       Date:  2022-05-21

3.  In vitro and in vivo activities of 2-aminopyrazines and 2-aminopyridines in experimental models of human African trypanosomiasis.

Authors:  Suman K Vodnala; Thomas Lundbäck; Birger Sjöberg; Richard Svensson; Martin E Rottenberg; Lars G J Hammarström
Journal:  Antimicrob Agents Chemother       Date:  2012-12-17       Impact factor: 5.191

4.  DNA mimics for the rapid identification of microorganisms by fluorescence in situ hybridization (FISH).

Authors:  Laura Cerqueira; Nuno F Azevedo; Carina Almeida; Tatiana Jardim; Charles William Keevil; Maria J Vieira
Journal:  Int J Mol Sci       Date:  2008-10-20       Impact factor: 5.923

Review 5.  Dourine: a neglected disease of equids.

Authors:  Yonas Gizaw; Mulisa Megersa; Teka Fayera
Journal:  Trop Anim Health Prod       Date:  2017-04-24       Impact factor: 1.559
  5 in total

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