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Literature DB >> 12409378

Method for inactivating and fixing unstained smear preparations of mycobacterium tuberculosis for improved laboratory safety.

Pamela Chedore¹, Cecelia Th'ng, Dennis H Nolan, George M Churchwell, David E Sieffert, Yvonne M Hale, Frances Jamieson.

Abstract

The inactivation of smears that contain Mycobacterium tuberculosis for microscopy before removal of the material from a biosafety cabinet is an important safety factor in preventing the potential transmission of tuberculosis to laboratory workers. The fixing and inactivating properties of heat flaming, 70% ethanol, and 1, 3, and 5% phenol in ethanol for smears containing M. tuberculosis were investigated. Heat flaming failed to inactivate the smear material, whereas 5% phenol in ethanol successfully fixed and inactivated all smears containing M. tuberculosis both from concentrated sputum samples and from culture material.

Entities: Chemical Species

Mesh：

Substances：
Fixatives
Phenol
Ethanol

Year: 2002 PMID： 12409378 PMCID： PMC139704 DOI： 10.1128/JCM.40.11.4077-4080.2002

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

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