| Literature DB >> 12409016 |
Abstract
Soluble peroxidase (POD) from oil palm leaf was purified by (NH(4))(2)SO(4) precipitation, anion exchange chromatography and molecular exclusion chromatography. The purification grade obtained was 429 yielding 54% of the enzyme activity. Electrophoresis of purified enzyme under denatured conditions revealed M(r) of 48+/-2 kDa. It has an optimum pH of 5 and it exhibited very high pH and thermal stabilities. K(m) for guaiacol, ABTS and pyrogallol were 3.96, 1 and 0.84 mM, respectively. Immunocytochemical localization studies showed that soluble POD was mainly located in the vascular bundles and epidermis of leaf.Entities:
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Year: 2002 PMID： 12409016 DOI： 10.1016/s0031-9422(02)00167-x
Source DB: PubMed Journal: Phytochemistry ISSN： 0031-9422 Impact factor: 4.072