Literature DB >> 12405355

Expression and folding of an antibody fragment selected in vivo for high expression levels in Escherichia coli cytoplasm.

Jean-Charles Laden1, Pascal Philibert, François Torreilles, Martine Pugnière, Pierre Martineau.   

Abstract

In this review, we summarize some of our results on folding and directed evolution of an antibody fragment in Escherichia coli cytoplasm. We will also discuss some attempts to construct other antibodies active in this cellular compartment.

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Year:  2002        PMID： 12405355     DOI： 10.1016/s0923-2508(02)01347-5

Source DB:  PubMed          Journal:  Res Microbiol        ISSN： 0923-2508            Impact factor:   3.992


  6 in total

1.  Efficient isolation of soluble intracellular single-chain antibodies using the twin-arginine translocation machinery.

Authors:  Adam C Fisher; Matthew P DeLisa
Journal:  J Mol Biol       Date:  2008-11-01       Impact factor: 5.469

2.  Prokaryotic expression and renaturation of engineering chimeric Fab antibody against human hepatoma.

Authors:  Jin-Liang Xing; Xiang-Min Yang; Xi-Ying Yao; Fei Song; Zhi-Nan Chen
Journal:  World J Gastroenterol       Date:  2004-07-15       Impact factor: 5.742

3.  A focused antibody library for selecting scFvs expressed at high levels in the cytoplasm.

Authors:  Pascal Philibert; Audrey Stoessel; Wei Wang; Annie-Paule Sibler; Nicole Bec; Christian Larroque; Jeffery G Saven; Jérôme Courtête; Etienne Weiss; Pierre Martineau
Journal:  BMC Biotechnol       Date:  2007-11-22       Impact factor: 2.563

4.  Directed evolution of single-chain Fv for cytoplasmic expression using the beta-galactosidase complementation assay results in proteins highly susceptible to protease degradation and aggregation.

Authors:  Pascal Philibert; Pierre Martineau
Journal:  Microb Cell Fact       Date:  2004-12-17       Impact factor: 5.328

5.  Molecular mechanism of antibody-mediated activation of β-galactosidase.

Authors:  Kutti R Vinothkumar; Greg McMullan; Richard Henderson
Journal:  Structure       Date:  2014-03-06       Impact factor: 5.006

Review 6.  Antibody-mediated enzyme formation: Its legacy at age fifty-four.

Authors:  Roberto Strom; Franco Celada
Journal:  J Mol Recognit       Date:  2021-10-24       Impact factor: 2.891
  6 in total

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