| Literature DB >> 12399046 |
Sun Mi Lee1, Gregor Grass, Christopher J Haney, Bin Fan, Barry P Rosen, Andreas Anton, Dietrich H Nies, Christopher Rensing.
Abstract
The membrane transporter ZitB responsible for Zn(II) efflux in Escherichia coli was studied by site-directed mutagenesis to elucidate the function of individual amino acid residues. Substitutions of several charged or polar residues, H53, H159, D163 and D186, located in predicted transmembrane domains resulted in loss of ZitB function. In contrast, neither the amino-terminal nor the carboxy-terminal regions, both histidine-rich, were required for function.Entities:
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Year: 2002 PMID： 12399046 DOI： 10.1111/j.1574-6968.2002.tb11402.x
Source DB: PubMed Journal: FEMS Microbiol Lett ISSN： 0378-1097 Impact factor: 2.742