| Literature DB >> 12398352 |
Donghai Lin1, Kong Hung Sze, Yangfang Cui, Guang Zhu.
Abstract
The recent introduction of the SEA-TROSY experiment (Pellecchia et al. (2001) J. Am. Chem. Soc., 123, 4633-4634) can alleviate the problem of resonance overlap in 15N/2H labeled proteins. This method selectively observes solvent exposed amide protons with a SEA element. However, SEA-TROSY spectra may be contaminated with exchange-relayed NOE contributions from fast exchanging hydroxyl or amine protons and longitudinal relaxation contributions. Furthermore, for non-deuterated proteins or protein-ligand complexes, SEA-TROSY spectra may contain NOE contributions from aliphatic protons. In this communication, a modified version of the SEA element, a Clean SEA element, is introduced to eliminate these artifacts.Entities:
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Year: 2002 PMID： 12398352 DOI： 10.1023/a:1020225206644
Source DB: PubMed Journal: J Biomol NMR ISSN： 0925-2738 Impact factor: 2.835