| Literature DB >> 12393815 |
Marialuisa Lavitrano1, Maria Laura Bacci, Monica Forni, Davide Lazzereschi, Carla Di Stefano, Daniela Fioretti, Paola Giancotti, Gabriella Marfé, Loredana Pucci, Luigina Renzi, Hongjun Wang, Antonella Stoppacciaro, Giorgio Stassi, Massimo Sargiacomo, Paola Sinibaldi, Valeria Turchi, Roberto Giovannoni, Giacinto Della Casa, Eraldo Seren, Giancarlo Rossi.
Abstract
A large number of hDAF transgenic pigs to be used for xenotransplantation research were generated by using sperm-mediated gene transfer (SMGT). The efficiency of transgenesis obtained with SMGT was much greater than with any other method. In the experiments reported, up to 80% of pigs had the transgene integrated into the genome. Most of the pigs carrying the hDAF gene transcribed it in a stable manner (64%). The great majority of pigs that transcribed the gene expressed the protein (83%). The hDAF gene was transmitted to progeny. Expression was stable and found in caveolae as it is in human cells. The expressed gene was functional based on in vitro experiments performed on peripheral blood mononuclear cells. These results show that our SMGT approach to transgenesis provides an efficient procedure for studies involving large animal models.Entities:
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Year: 2002 PMID： 12393815 PMCID： PMC137866 DOI： 10.1073/pnas.222550299
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205