Effect of Candida albicans metabolite(s) on cellular actin.

The present study describes experiments of the effect of Candida albicans metabolite(s) (arcsf) on cellular actin, and constitutes a continuation of our previous investigations regarding the effect of the fungus on actin. Western blot analysis of HEp2 cells exposed to arcsf or subfraction of arcsf (<100 kDa) revealed that the amount of soluble actin was decreased, and total actin increased, as compared to untreated cells. Transmission electron microscopy observations of HEp2 cells interacted with C. albicans or arcsf, and gold-labeled specifically for actin, revealed more intensive labeling, with labeled particles clustered in groups. Confocal laser scanning microscopy analysis has shown, as well, that exposure of HEp2 cells to the <100-kDa subfraction resulted in actin rearrangement, as did the unfractionated arcsf. Our results point to: (1) C. albicans metabolite(s) affects cellular actin by increasing the transition of soluble actin to the insoluble form; (2) the effect is associated with the subfraction of arcsf which is smaller than <100 kDa.