OBJECTIVES: The purpose of this study was to elucidate whether the neuronal noradrenaline reuptake transporter (uptake1) undergoes age-dependent regulation in the human heart. BACKGROUND: Aging is associated with various alterations in cardiovascular function. METHODS: We determined uptake1 density (by [3H]-nisoxetine binding to membranes) and activity (by accumulation of [3H]-noradrenaline into tissue slices) in the right atria (RA) of 42 patients (age range 3 months to 76 years) undergoing open-heart surgery without apparent heart failure. Moreover, the effects of 1 micromol/l desipramine on the noradrenaline-induced positive inotropic effect were assessed in the isolated, electrically driven RA trabeculae of these patients. RESULTS: There was a significant negative correlation between RA uptake1 density and age; moreover, RA uptake1 activity was significantly reduced in elderly patients. Desipramine (1 micromol/l) significantly shifted noradrenaline concentration-response curves to the left; this shift was significantly more pronounced in younger patients than in older patients. CONCLUSIONS: With increasing age, human myocardial uptake1 activity decreases, possibly because of age-dependent downregulation of uptake1 density.
OBJECTIVES: The purpose of this study was to elucidate whether the neuronal noradrenaline reuptake transporter (uptake1) undergoes age-dependent regulation in the human heart. BACKGROUND: Aging is associated with various alterations in cardiovascular function. METHODS: We determined uptake1 density (by [3H]-nisoxetine binding to membranes) and activity (by accumulation of [3H]-noradrenaline into tissue slices) in the right atria (RA) of 42 patients (age range 3 months to 76 years) undergoing open-heart surgery without apparent heart failure. Moreover, the effects of 1 micromol/l desipramine on the noradrenaline-induced positive inotropic effect were assessed in the isolated, electrically driven RA trabeculae of these patients. RESULTS: There was a significant negative correlation between RA uptake1 density and age; moreover, RA uptake1 activity was significantly reduced in elderly patients. Desipramine (1 micromol/l) significantly shifted noradrenaline concentration-response curves to the left; this shift was significantly more pronounced in younger patients than in older patients. CONCLUSIONS: With increasing age, human myocardial uptake1 activity decreases, possibly because of age-dependent downregulation of uptake1 density.
Authors: Arnold F Jacobson; Ji Chen; Liudmilla Verdes; Russell D Folks; Daya N Manatunga; Ernest V Garcia Journal: J Nucl Cardiol Date: 2013-03-13 Impact factor: 5.952
Authors: Yu-Shin Ding; Tarun Singhal; Beata Planeta-Wilson; Jean-Dominique Gallezot; Nabeel Nabulsi; David Labaree; Jim Ropchan; Shannan Henry; Wendol Williams; Richard E Carson; Alexander Neumeister; Robert T Malison Journal: Synapse Date: 2010-01 Impact factor: 2.562
Authors: Marco Mongillo; Anna S John; Lucia Leccisotti; Dudley J Pennell; Paolo G Camici Journal: Eur J Nucl Med Mol Imaging Date: 2007-02-10 Impact factor: 10.057
Authors: Anna S John; Marco Mongillo; Christophe Depre; Muhammad T Khan; Ornella E Rimoldi; John R Pepper; Gilles D Dreyfus; Dudley J Pennell; Paolo G Camici Journal: Eur J Nucl Med Mol Imaging Date: 2007-07-28 Impact factor: 9.236