OBJECTIVE: The purpose of this study was to determine the effect of locally applied platelet-activating factor on cervical ripening and leukocyte infiltration. STUDY DESIGN: Timed-pregnant rats were treated vaginally twice each day on days 14 and 15 of gestation with platelet-activating factor 5 x 10(-8) mol/L (low-dose), 5 x 10(-7) mol/L (high-dose), or solvent and were killed on day 16. Cervical ripening was assessed by cervical resistance-to-stretch and light-induced autofluorescence of cross-linked collagen. Longitudinal sections of the cervix were hematoxylin and eosin stained, and the number of polymorphonuclear leukocytes in eight high-power fields was determined. RESULTS: Cervical resistance-to-stretch and light-induced autofluorescence were decreased in rats that were treated with high-dose platelet-activating factor compared with controls (0.4375 +/- 0.0126 N/mm vs 0.5528 +/- 0.0294 N/mm, and 2.739 +/- 0.150 N/mm vs 3.475 +/- 0.236 N/mm; P <.05). High-dose platelet-activating factor led to infiltration with polymorphonuclear leukocytes compared with control (22.1 +/- 5.2 vs 2.1 +/- 1.3 cells/8 high-power fields, P <.05). CONCLUSION: Local platelet-activating factor application induces cervical ripening accompanied by stromal polymorphonuclear leukocyte infiltration. Platelet-activating factor may play a major role in parturition under physiologic and pathologic conditions.
OBJECTIVE: The purpose of this study was to determine the effect of locally applied platelet-activating factor on cervical ripening and leukocyte infiltration. STUDY DESIGN: Timed-pregnant rats were treated vaginally twice each day on days 14 and 15 of gestation with platelet-activating factor 5 x 10(-8) mol/L (low-dose), 5 x 10(-7) mol/L (high-dose), or solvent and were killed on day 16. Cervical ripening was assessed by cervical resistance-to-stretch and light-induced autofluorescence of cross-linked collagen. Longitudinal sections of the cervix were hematoxylin and eosin stained, and the number of polymorphonuclear leukocytes in eight high-power fields was determined. RESULTS: Cervical resistance-to-stretch and light-induced autofluorescence were decreased in rats that were treated with high-dose platelet-activating factor compared with controls (0.4375 +/- 0.0126 N/mm vs 0.5528 +/- 0.0294 N/mm, and 2.739 +/- 0.150 N/mm vs 3.475 +/- 0.236 N/mm; P <.05). High-dose platelet-activating factor led to infiltration with polymorphonuclear leukocytes compared with control (22.1 +/- 5.2 vs 2.1 +/- 1.3 cells/8 high-power fields, P <.05). CONCLUSION: Local platelet-activating factor application induces cervical ripening accompanied by stromal polymorphonuclear leukocyte infiltration. Platelet-activating factor may play a major role in parturition under physiologic and pathologic conditions.
Authors: Ruben J Kuon; Shao-Qing Shi; Holger Maul; Christof Sohn; James Balducci; William L Maner; Robert E Garfield Journal: Am J Obstet Gynecol Date: 2010-05 Impact factor: 8.661
Authors: Lu Gao; Elizabeth H Rabbitt; Jennifer C Condon; Nora E Renthal; John M Johnston; Matthew A Mitsche; Pierre Chambon; Jianming Xu; Bert W O'Malley; Carole R Mendelson Journal: J Clin Invest Date: 2015-06-22 Impact factor: 14.808
Authors: Hanan H Wahid; Peck Yin Chin; David J Sharkey; Kerrilyn R Diener; Mark R Hutchinson; Kenner C Rice; Lachlan M Moldenhauer; Sarah A Robertson Journal: Am J Pathol Date: 2020-02-18 Impact factor: 4.307