| Literature DB >> 12381367 |
Vladimír Mastihuba1, Lubomír Kremnický, Mária Mastihubová, J L Willett, Gregory L Côté.
Abstract
We have developed a spectrophotometric assay for the quantitative determination of feruloyl esterase activity based on release of 4-nitrophenol from a novel substrate, 4-nitrophenyl ferulate in an emulsion of Triton X-100 in aqueous buffer solution. The release of 4-nitrophenol was linear with reaction time at an early stage of the reaction with various esterase preparations. The method proposed here is accurate, rapid, and easy to perform.Entities:
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Year: 2002 PMID: 12381367 DOI: 10.1016/s0003-2697(02)00241-5
Source DB: PubMed Journal: Anal Biochem ISSN: 0003-2697 Impact factor: 3.365